ArticleProceedings of the National Academy of Sciences of the United States of America2009
Diatom plastids depend on nucleotide import from the cytosol.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 83 citations in OpenAlex.
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
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- Array of metabolic pathways in a kleptoplastidic foraminiferan protist supports chemoautotrophy in dark, euxinic seafloor sediments.The ISME journal · 2025Article
- Photosynthetic directed endosymbiosis to investigate the role of bioenergetics in chloroplast function and evolution.Nature communications · 2024Article
- Prey preference in a kleptoplastic dinoflagellate is linked to photosynthetic performance.The ISME journal · 2023Article
- Discovery, implications and initial use of semi-synthetic organisms with an expanded genetic alphabet/code.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Review
- From polymerase engineering to semi-synthetic life: artificial expansion of the central dogma.RSC chemical biology · 2022Review
- A metabolic, phylogenomic and environmental atlas of diatom plastid transporters from the model speciesFrontiers in plant science · 2022Article
- The Tapetal Major Facilitator NPF2.8 Is Required for Accumulation of Flavonol Glycosides on the Pollen Surface inThe Plant cell · 2020Article
- Sequencing and Analysis of the Complete Organellar Genomes ofFrontiers in plant science · 2020Article
- Mobilization and Cellular Distribution of Phosphate in the DiatomFrontiers in plant science · 2020Article
- Nucleotide Transport and Metabolism in Diatoms.Biomolecules · 2019Review
- Organelle Studies and Proteome Analyses of Mitochondria and Plastids Fractions from the Diatom Thalassiosira pseudonana.Plant & cell physiology · 2019Article
- Optimization of Replication, Transcription, and Translation in a Semi-Synthetic Organism.Journal of the American Chemical Society · 2019Article
- Carbon Orientation in the DiatomFrontiers in plant science · 2019Article
- Progress Toward a Semi-Synthetic Organism with an Unrestricted Expanded Genetic Alphabet.Journal of the American Chemical Society · 2018Article
- Expansion of the genetic code via expansion of the genetic alphabet.Current opinion in chemical biology · 2018Review
- Mitochondrial Glycolysis in a Major Lineage of Eukaryotes.Genome biology and evolution · 2018Article
- Ion and metabolite transport in the chloroplast of algae: lessons from land plants.Cellular and molecular life sciences : CMLS · 2018Review
- A Tool for the Import of Natural and Unnatural Nucleoside Triphosphates into Bacteria.Journal of the American Chemical Society · 2018Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Diatoms are ecologically important algae that acquired their plastids by secondary endosymbiosis, resulting in a more complex cell structure and an altered distribution of metabolic pathways when compared with organisms with primary plastids. Diatom plastids are surrounded by 4 membranes; the outermost membrane is continuous with the endoplasmic reticulum. Genome analyses suggest that nucleotide biosynthesis is, in contrast to higher plants, not located in the plastid, but in the cytosol. As a consequence, nucleotides have to be imported into the organelle. However, the mechanism of nucleotide entry into the complex plastid is unknown. We identified a high number of putative nucleotide transporters (NTTs) in the diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum and characterized the first 2 isoforms (NTT1 and NTT2). GFP-based localization studies revealed that both investigated NTTs are targeted to the plastid membranes, and that NTT1 most likely enters the innermost plastid envelope via the stroma. Heterologously expressed NTT1 acts as a proton-dependent adenine nucleotide importer, whereas NTT2 facilitates the counter exchange of (deoxy-)nucleoside triphosphates. Therefore, these transporters functionally resemble NTTs from obligate intracellular bacteria with an impaired nucleotide metabolism rather than ATP/ADP exchanging NTTs from primary plastids. We suggest that diatoms harbor a specifically-adapted nucleotide transport system and that NTTs are the key players in nucleotide supply to the complex plastid.
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