ArticleVirology journal2009
Intrinsic disorder in Viral Proteins Genome-Linked: experimental and predictive analyses.
Article in Virology journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 70 citations in OpenAlex.
- The C-terminal Domain of SARS-CoV-2 nsp8 is a Molten Globule in the Absence of Binding Partners.Journal of molecular biology · 2025Article
- The Disorderly Nature of Caliciviruses.Viruses · 2024Review
- VPg Impact on Ryegrass Mottle Virus Serine-like 3C Protease Proteolysis and Structure.International journal of molecular sciences · 2023Article
- Polerovirus genomic variation.Virus evolution · 2021Article
- Genome-Wide Variation in Betacoronaviruses.Journal of virology · 2021Article
- Article
- Plant virus evolution under strong drought conditions results in a transition from parasitism to mutualism.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Functional Characterization of Pepper Vein Banding Virus-Encoded Proteins and Their Interactions: Implications inViruses · 2020Review
- Intrinsically disordered proteins of viruses: Involvement in the mechanism of cell regulation and pathogenesis.Progress in molecular biology and translational science · 2020Review
- Structural studies of the eIF4E-VPg complex reveal a direct competition for capped RNA: Implications for translation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Hydrodynamic Behavior of the Intrinsically Disordered Potyvirus Protein VPg, of the Translation Initiation Factor eIF4E and of their Binary Complex.International journal of molecular sciences · 2019Article
- Genome-Wide Variation in Potyviruses.Frontiers in plant science · 2019Article
- Comparative analysis of mutational robustness of the intrinsically disordered viral protein VPg and of its interactor eIF4E.PloS one · 2019Article
- First Experimental Assessment of Protein Intrinsic Disorder Involvement in an RNA Virus Natural Adaptive Process.Molecular biology and evolution · 2018Article
- The Potyvirus Silencing Suppressor Protein VPg Mediates Degradation of SGS3 via Ubiquitination and Autophagy Pathways.Journal of virology · 2017Article
- Article
- Plant Translation Factors and Virus Resistance.Viruses · 2015Review
- Overview on Sobemoviruses and a Proposal for the Creation of the Family Sobemoviridae.Viruses · 2015Review
- Interaction patterns between potato virus Y and eIF4E-mediated recessive resistance in the Solanaceae.Journal of virology · 2014Article
- A short motif in the N-terminal part of the coat protein is a host-specific determinant of systemic infectivity for two potyviruses.Molecular plant pathology · 2014Article
Corrections and comments
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Authors and funding
11 authors at 9 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVPgs are viral proteins linked to the 5' end of some viral genomes. Interactions between several VPgs and eukaryotic translation initiation factors eIF4Es are critical for plant infection. However, VPgs are not restricted to phytoviruses, being also involved in genome replication and protein translation of several animal viruses. To date, structural data are still limited to small picornaviral VPgs. Recently three phytoviral VPgs were shown to be natively unfolded proteins.
resultsIn this paper, we report the bacterial expression, purification and biochemical characterization of two phytoviral VPgs, namely the VPgs of Rice yellow mottle virus (RYMV, genus Sobemovirus) and Lettuce mosaic virus (LMV, genus Potyvirus). Using far-UV circular dichroism and size exclusion chromatography, we show that RYMV and LMV VPgs are predominantly or partly unstructured in solution, respectively. Using several disorder predictors, we show that both proteins are predicted to possess disordered regions. We next extend theses results to 14 VPgs representative of the viral diversity. Disordered regions were predicted in all VPg sequences whatever the genus and the family.
conclusionBased on these results, we propose that intrinsic disorder is a common feature of VPgs. The functional role of intrinsic disorder is discussed in light of the biological roles of VPgs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.