ArticleMolecular and cellular biology1991
The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 103 citations in OpenAlex.
- Cell integrity limits ploidy in budding yeast.G3 (Bethesda, Md.) · 2025Article
- CDC20: a novel therapeutic target in cancer.American journal of translational research · 2023Review
- The anaphase-promoting complex regulates the degradation of the inner nuclear membrane protein Mps3.The Journal of cell biology · 2019Article
- Histone H3K4 methylation regulates deactivation of the spindle assembly checkpoint through direct binding of Mad2.Genes & development · 2016Article
- The mitotic checkpoint complex (MCC): looking back and forth after 15 years.AIMS molecular science · 2016Article
- Active transport can greatly enhance Cdc20:Mad2 formation.International journal of molecular sciences · 2014Article
- Spatial rule-based modeling: a method and its application to the human mitotic kinetochore.Cells · 2013Article
- Rereplication in emi1-deficient zebrafish embryos occurs through a Cdh1-mediated pathway.PloS one · 2012Article
- Regulated degradation of the APC coactivator Cdc20.Cell division · 2010Article
- The mother enrichment program: a genetic system for facile replicative life span analysis in Saccharomyces cerevisiae.Genetics · 2009Article
- Analysis of the mitotic exit control system using locked levels of stable mitotic cyclin.Molecular systems biology · 2009Article
- Explorative data analysis of MCL reveals gene expression networks implicated in survival and prognosis supported by explorative CGH analysis.BMC cancer · 2008Article
- Analysis of a generic model of eukaryotic cell-cycle regulation.Biophysical journal · 2006Article
- Regulation of the cell cycle by protein phosphatase 2A in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2006Review
- Essential tension and constructive destruction: the spindle checkpoint and its regulatory links with mitotic exit.The Biochemical journal · 2005Review
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- Article
- Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Fission yeast Fizzy-related protein srw1p is a G(1)-specific promoter of mitotic cyclin B degradation.The EMBO journal · 2000Article
- Cdc28 activates exit from mitosis in budding yeast.The Journal of cell biology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Previous analysis of cdc20 mutants of the yeast Saccharomyces cerevisiae suggests that the CDC20 gene product (Cdc20p) is required for two microtubule-dependent processes, nuclear movements prior to anaphase and chromosome separation. Here we report that cdc20 mutants are defective for a third microtubule-mediated event, nuclear fusion during mating of G1 cells, but appear normal for a fourth microtubule-dependent process, nuclear migration after DNA replication. Therefore, Cdc20p is required for a subset of microtubule-dependent processes and functions at multiple stages in the life cycle. Consistent with this interpretation, we find that cdc20 cells arrested by alpha-factor or at the restrictive temperature accumulate anomalous microtubule structures, as detected by indirect immunofluorescence. The anomalous microtubule staining patterns are due to cdc20 because intragenic revertants that revert the temperature sensitivity have normal microtubule morphologies. cdc20 mutants have a sevenfold increase in the intensity of antitubulin fluorescence in intranuclear spindles compared with spindles from wild-type cells, yet the total amount of tubulin is indistinguishable by Western immunoblot analysis. This result suggests that Cdc20p modulates microtubule structure in wild-type cells either by promoting microtubule disassembly or by altering the surface of the microtubules. Finally, we cloned and sequenced CDC20 and show that it encodes a member of a family of proteins that share homology to the beta subunit of transducin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.