ArticleMolecular and cellular biology1991
Identification and characterization of a T-cell-specific enhancer adjacent to the murine CD4 gene.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 116 citations in OpenAlex.
- A Bcl11bFrontiers in immunology · 2024Article
- Epigenetic Reprogramming Leads to Downregulation of CD4 and Functional Changes in African Green Monkey Memory CD4Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Epigenetic regulation of T cell adaptive immunity.Immunological reviews · 2021Review
- Epigenetic silencing of CD4 expression in nonpathogenic SIV infection in African green monkeys.JCI insight · 2020Article
- Global dynamics of stage-specific transcription factor binding during thymocyte development.Scientific reports · 2018Article
- Heritable Gene Regulation in the CD4:CD8 T Cell Lineage Choice.Frontiers in immunology · 2017Review
- What Happens in the Thymus Does Not Stay in the Thymus: How T Cells Recycle the CD4+-CD8+ Lineage Commitment Transcriptional Circuitry To Control Their Function.Journal of immunology (Baltimore, Md. : 1950) · 2016Review
- Rapid and unambiguous detection of DNase I hypersensitive site in rare population of cells.PloS one · 2014Article
- Transcriptional control of CD4 and CD8 coreceptor expression during T cell development.Cellular and molecular life sciences : CMLS · 2013Review
- The epigenetic landscape of lineage choice: lessons from the heritability of CD4 and CD8 expression.Current topics in microbiology and immunology · 2012Review
- Transcription factor AP4 modulates reversible and epigenetic silencing of the Cd4 gene.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Transcription initiation platforms and GTF recruitment at tissue-specific enhancers and promoters.Nature structural & molecular biology · 2011Article
- Epigenetic propagation of CD4 expression is established by the Cd4 proximal enhancer in helper T cells.Genes & development · 2010Article
- Regulation of p110delta PI 3-kinase gene expression.PloS one · 2009Article
- Id1 induces apoptosis through inhibition of RORgammat expression.BMC immunology · 2008Article
- Development of all CD4 T lineages requires nuclear factor TOX.The Journal of experimental medicine · 2008Article
- Negative regulation of CD8 expression via Cd8 enhancer-mediated recruitment of the zinc finger protein MAZR.Nature immunology · 2006Article
- A reliable method to display authentic DNase I hypersensitive sites at long-ranges in single-copy genes from large genomes.Nucleic acids research · 2006Article
- Runx1 binds positive transcription elongation factor b and represses transcriptional elongation by RNA polymerase II: possible mechanism of CD4 silencing.Molecular and cellular biology · 2005Article
- Immunopathogenesis of oropharyngeal candidiasis in human immunodeficiency virus infection.Clinical microbiology reviews · 2004Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Expression of the CD4 and CD8 glycoproteins is a tightly regulated process tied to the maturation of functionally distinct classes of thymocytes. Therefore, understanding of the mechanism of expression of the genes encoding CD4 and CD8 is likely to yield important insight into regulation of the differentiated functions of T cells. Here, we report the identification of a T-cell-specific enhancer in a DNase I-hypersensitive region about 13 kb 5' of the transcription initiation site of the murine CD4 gene. Within the minimal enhancer element, at least three nuclear protein binding sites were identified by DNase I footprint analysis. One site contains the consensus motif for TCF-1 alpha/LEF-1, a recently identified HMG box transcription factor primarily expressed in pre-B and T cells. By Southwestern (DNA-protein) blotting and binding competition analyses, the protein binding to this site was found to be indistinguishable from TCF-1 alpha/LEF-1. Mutagenesis of this site resulted in loss of factor binding but had a relatively minor effect on enhancer activity. In contrast, mutations in another site, containing two consensus binding motifs for basic helix-loop-helix proteins, abolished factor binding and dramatically reduced enhancer activity. None of the protein binding sites had activity on its own, suggesting that the CD4 enhancer requires the interaction of multiple regulatory sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.