ArticleMolecular and cellular biology1991
Activation of skeletal alpha-actin gene transcription: the cooperative formation of serum response factor-binding complexes over positive cis-acting promoter serum response elements displaces a negative-acting nuclear factor enriched in replicating myoblasts and nonmyogenic cells.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 142 citations in OpenAlex.
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- Competitive binding between Seryl-tRNA synthetase/YY1 complex and NFKB1 at the distal segment results in differential regulation of human vegfa promoter activity during angiogenesis.Nucleic acids research · 2017Article
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- The STARS signaling pathway: a key regulator of skeletal muscle function.Pflugers Archiv : European journal of physiology · 2014Review
- miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors.Orphanet journal of rare diseases · 2013Article
- In vivo fate mapping identifies mesenchymal progenitor cells.Stem cells (Dayton, Ohio) · 2012Article
- Preferential skeletal muscle myosin loss in response to mechanical silencing in a novel rat intensive care unit model: underlying mechanisms.The Journal of physiology · 2011Article
- Myocardin-related transcription factor A is a common mediator of mechanical stress- and neurohumoral stimulation-induced cardiac hypertrophic signaling leading to activation of brain natriuretic peptide gene expression.Molecular and cellular biology · 2010Article
- KLF3 regulates muscle-specific gene expression and synergizes with serum response factor on KLF binding sites.Molecular and cellular biology · 2010Article
- Binding of serum response factor to cystic fibrosis transmembrane conductance regulator CArG-like elements, as a new potential CFTR transcriptional regulation pathway.Nucleic acids research · 2005Article
- Serum response factor plays an important role in the mechanically overloaded plantaris muscle of rats.Histochemistry and cell biology · 2003Article
- Serum response factor is crucial for actin cytoskeletal organization and focal adhesion assembly in embryonic stem cells.The Journal of cell biology · 2002Article
- The role of interleukin-1 in the failing heart.Heart failure reviews · 2001Review
- Increased level and duration of expression in muscle by co-expression of a transactivator using plasmid systems.Gene therapy · 1999Article
- Activation of the skeletal alpha-actin promoter during muscle regeneration.Journal of muscle research and cell motility · 1998Article
- Regulation of serum amyloid A protein expression during the acute-phase response.The Biochemical journal · 1998Review
- Influence of promoter potency on the transcriptional effects of YY1, SRF and Msx-1 in transient transfection analysis.Nucleic acids research · 1998Article
- Molecular cloning of a novel mouse gene with predominant muscle and neural expression.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- Cell-specific transcription of the smooth muscle gamma-actin gene requires both positive- and negative-acting cis elements.Gene expression · 1998Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Three upstream CBAR cis-acting promoter elements, containing the inner core CC(A/T)6GG of the serum response element (SRE), are required for myogenic cell type-restricted expression of the avian skeletal alpha-actin gene (K.L. Chow and R.J. Schwartz, Mol. Cell. Biol. 10:528-538, 1990). These actin SRE elements display differential binding properties with two distinct nuclear proteins, serum response factor (SRF) and another factor described here as F-ACT1. SRF is able to bind to all actin SREs with various affinities. This multisite interaction is marked by cooperative binding events in that the two high-affinity proximal and distal SREs facilitate the weak central-site interaction with SRF, leading to the formation of a higher-order SRF-promoter complex. Functional analyses reveal that undisrupted multiple SRF-DNA interactions are absolutely essential for promoter activity in myogenic cells. F-ACT1, present at higher levels in nonmyogenic cells and replicating myoblasts than in myotubes, binds solely to the proximal SRE, and its binding is mutually exclusive with that of SRF owing to their overlapping base contacts. The cooperative promoter binding by SRF, however, can effectively displace prebound F-ACT1. In addition, an intact F-ACT1 binding site acts as a negative promoter element by restricting developmentally timed expression in myoblasts. F-ACT1 may therefore act as a repressor of skeletal alpha-actin gene transcription. This interplay between F-ACT1 and SRF may constitute a developmental as well as a physiologically regulated mechanism which modulates sarcomeric actin gene expression.
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