ArticleEndocrine2009
Estrogen modulates expression of putative housekeeping genes in the mouse uterus.
Article in Endocrine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Transcriptomic Profiling Reveals Urokinase-Type Plasminogen Activator-Mediated Regulation of Metabolic Competence and Cumulus Expansion During Mouse Oocyte In Vitro Maturation.International journal of molecular sciences · 2026Article
- Comprehensive Genetic and Molecular Characterization Confirms Hepatic Stellate Cell Origin of the Immortal Col-GFP HSC Line.International journal of molecular sciences · 2025Article
- Reference gene selection in bovine caruncular epithelial cells under pregnancy-associated hormones exposure.Scientific reports · 2022Article
- Identification of Reference Genes for Circadian Studies on Brain Microvessels and Choroid Plexus Samples Isolated from Rats.Biomolecules · 2021Article
- Identification and validation of suitable reference genes for quantitative real-time PCR gene expression analysis in pregnant human myometrium.Molecular biology reports · 2021Article
- Uterine aquaporin expression is dynamically regulated by estradiol and progesterone and ovarian stimulation disrupts embryo implantation without affecting luminal closure.Molecular human reproduction · 2020Article
- Antiestrogens in combination with immune checkpoint inhibitors in breast cancer immunotherapy.The Journal of steroid biochemistry and molecular biology · 2019Article
- Use of the REVERTMolecular and cellular endocrinology · 2018Article
- Sex Hormones RegulateFrontiers in molecular neuroscience · 2018Article
- Changes in ambient temperature elicit divergent control of metabolic and cardiovascular actions by leptin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Differential protein expression profile in the hypothalamic GT1-7 cell line after exposure to anabolic androgenic steroids.PloS one · 2017Article
- Developmental exposures to bisphenol S, a BPA replacement, alter estrogen-responsiveness of the female reproductive tract: a pilot study.Cogent medicine · 2017Article
- Urolithin A suppresses the proliferation of endometrial cancer cells by mediating estrogen receptor-α-dependent gene expression.Molecular nutrition & food research · 2016Article
- Diet-induced obesity impairs endometrial stromal cell decidualization: a potential role for impaired autophagy.Human reproduction (Oxford, England) · 2016Article
- Deletion of Arid1a in Reproductive Tract Mesenchymal Cells Reduces Fertility in Female Mice.Biology of reproduction · 2016Article
- Article
- The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells.BMC research notes · 2013Article
- The development of cervical and vaginal adenosis as a result of diethylstilbestrol exposure in utero.Differentiation; research in biological diversity · 2012Review
- Effect of food restriction and leptin supplementation on fetal programming in mice.Endocrinology · 2012Article
- Selection of housekeeping genes for normalization of RT-PCR in hypoxic neural stem cells of rat in vitro.Molecular biology reports · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Estrogens regulate gene expression and cell proliferation in target tissues. In studies of estrogen-regulated gene expression, identification of appropriate housekeeping genes (HKGs), reference genes whose expression is not altered by treatment, is difficult. The goal of this study was to define HKGs unaltered by estrogen in the mouse uterus. Ovariectomized C57BL6 mice were dosed with 20 micrograms/kg ethinylestradiol and the uterus was collected at 6, 24, and 72 h later to bracket the biphasic time course of estrogen action in the rodent uterus. RNA was isolated, cDNA synthesized and equal amounts of cDNA were added to real-time PCR reactions. The expression of seven out of nine putative HKGs was altered by estrogen in the mouse uterus. Estrogen induced four gene expression profiles, expression of: (1) Actb and Hsp90ab1 were up-regulated early, (2) B2m and Gusb were up-regulated late, (3) Gapdh, Hprt1, and Ppia were up regulated at all time points, and (4) Rpl13a and 18srRNA were unaltered. This highlights the need to empirically determine the appropriate HKG for each experimental condition. Based on these results, we suggest using Rpl13a or 18srRNA as HKGs for xenoestrogen studies in the mouse uterus and as good candidates to test under different experimental conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.