Evidence map›Paper›PMID 19219499›Full record

ArticleCurrent microbiology2009

Metabolic shift in the phytopathogen Rhodococcus fascians in response to cell-free extract of infected tobacco plant tissues.

Laetitia Forizs, Sylvain Lestrade, Adeline Mol, Jean-François Dierick, Cécile Gerbaux, Billo Diallo, Mondher El Jaziri, Marie Baucher, Olivier M Vandeputte

Abstract read
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In one paragraph

Article in Current microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Proteome Analysis ofPlants (Basel, Switzerland) · 2023
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Laetitia ForizsLaboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, rue des Professeurs Jeener et Brachet 12, Gosselies, Belgium.
Sylvain Lestrade
Adeline Mol
Jean-François Dierick
Cécile Gerbaux
Billo Diallo
Mondher El Jaziri
Marie Baucher
Olivier M Vandeputte
Université Libre de Bruxelles · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The phytopathogen Rhodococcus fascians induces the development of leafy gall, which is considered to be its ecological niche. To obtain a view of the metabolic changes occurring in R. fascians during this process, an in vitro system was used where bacteria are grown in the presence of a leafy gall extract, a condition mimicking that found by the bacteria in infected plants. Proteins of R. fascians grown for 24 h under these conditions were displayed by two-dimensional polyacrylamide gel electrophoresis. Fifteen polypeptides showing a differential accumulation in response to the inducing conditions were analyzed by mass spectrometry. Two polypeptides potentially linked to the Krebs cycle, a pyruvate dehydrogenase and a fumarate hydratase, were further characterized and shown to be downregulated at the transcriptional level. The identification of these two enzymes suggests that R. fascians may shift its metabolism during the interaction with plants from the Krebs cycle to the glyoxylate shunt.

Indexed as

Host-Pathogen InteractionsBacterial ProteinsDNA, BacterialElectrophoresis, Gel, Two-DimensionalGene Expression ProfilingMass SpectrometryMolecular Sequence DataNicotianaPlant ExtractsProteomeRhodococcusSequence Analysis, DNABacterial ProteinsDNA, BacterialPlant ExtractsProteome

Identifiers

PMID19219499
OpenAlexW1970481195

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.