ArticleThe Journal of membrane biology2009
Mechanical force and cytoplasmic Ca(2+) activate yeast TRPY1 in parallel.
Article in The Journal of membrane biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- KCS1 and VIP1, the genes encoding yeast phosphoinositol pyrophosphate synthases, are required for CaFEBS open bio · 2025Article
- Calcium Ion Channels inJournal of fungi (Basel, Switzerland) · 2023Review
- Calmodulin kinase 2 genetically interacts with Rch1p to negatively regulate calcium import into Saccharomyces cerevisiae after extracellular calcium pulse.Archives of microbiology · 2022Article
- Structure of the ancient TRPY1 channel from Saccharomyces cerevisiae reveals mechanisms of modulation by lipids and calcium.Structure (London, England : 1993) · 2022Article
- Transient receptor potential channels: current perspectives on evolution, structure, function and nomenclature.Proceedings. Biological sciences · 2020Article
- Identification of Inhibitory CaiScience · 2019Article
- Article
- Calcium Transport Proteins in Fungi: The Phylogenetic Diversity of Their Relevance for Growth, Virulence, and Stress Resistance.Frontiers in microbiology · 2019Review
- SNARE-mediated membrane fusion arrests at pore expansion to regulate the volume of an organelle.The EMBO journal · 2018Article
- Calcium signaling and copper toxicity in Saccharomyces cerevisiae cells.Environmental science and pollution research international · 2016Article
- The Transient Receptor Potential (TRP) Channel Family in Colletotrichum graminicola: A Molecular and Physiological Analysis.PloS one · 2016Article
- Mechanical transduction by ion channels: A cautionary tale.World journal of neurology · 2015Article
- Activation of a TRP-like channel and intracellular Ca2+ dynamics during phospholipase-C-mediated cell death.Journal of cell science · 2014Article
- Molecular bases of multimodal regulation of a fungal transient receptor potential (TRP) channel.The Journal of biological chemistry · 2013Article
- Article
- The core domain as the force sensor of the yeast mechanosensitive TRP channel.The Journal of general physiology · 2011Article
- Pairing phosphoinositides with calcium ions in endolysosomal dynamics: phosphoinositides control the direction and specificity of membrane trafficking by regulating the activity of calcium channels in the endolysosomes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2011Review
- The transient receptor potential family of ion channels.Genome biology · 2011Review
- The use of yeast to understand TRP-channel mechanosensitivity.Pflugers Archiv : European journal of physiology · 2009Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The ability to sense mechanical and osmotic stimuli is vital to all organisms from mammals to bacteria. Members of the transient receptor potential (TRP) ion-channel family have attracted intense attention for their involvement in mechanosensation. The yeast homologue TRPY1 can clearly be activated by hypertonic shock in vivo and by stretch force under patch clamp. Like its animal counterparts, TRPY1 is polymodal, being gated by membrane stretch force and by cytoplasmic Ca(2+). Here, we investigated how these two gating principles interact. We found that stretch force can induce some channel activation without cytoplasmic Ca(2+). Tens of micromolar Ca(2+) greatly enhance the observed force-induced activities, with open probabilities following well the Boltzmann distribution, in which the two gating energies are summed as exponents. To map this formalism to structures, we found Ca(2+)-binding proteins such as calmodulin or calcineurin to be unnecessary. However, removing a dense cluster of negative charges in the C-terminal cytoplasmic domain of TRPY1 greatly diminishes the Ca(2+) activation as well as its influence on force activation. We also found a strategic point upstream of this charge cluster, at which insertion of amino acids weakens Ca(2+) activation considerably but leaves the mechanosensitivity nearly intact. These results led to a structure-function model in which Ca(2+) binding to the cytoplasmic domain and stretching of the membrane-embedded domain both generate gating force, reaching the gate in parallel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.