ArticleProceedings of the National Academy of Sciences of the United States of America2009
Evidence that family 35 carbohydrate binding modules display conserved specificity but divergent function.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 122 citations in OpenAlex.
- Nonspecific Binding of a Putative S-Layer Protein to Plant Cell Wall Polysaccharides-Implication for Growth Competence ofInternational journal of molecular sciences · 2025Article
- Identification of a novel xanthan-binding module of a multi-modularFrontiers in microbiology · 2024Article
- cazy_webscraper: local compilation and interrogation of comprehensive CAZyme datasets.Microbial genomics · 2023Article
- BoGH13ACellular and molecular life sciences : CMLS · 2023Article
- Identification and structural analysis of a carbohydrate-binding module specific to alginate, a representative of a new family, CBM96.The Journal of biological chemistry · 2023Article
- The structure of aJournal of structural biology: X · 2023Article
- Comparative genomics reveals cellobiose hydrolysis mechanism ofFrontiers in microbiology · 2022Article
- Carbohydrate-binding modules targeting branched polysaccharides: overcoming side-chain recalcitrance in a non-catalytic approach.Bioresources and bioprocessing · 2021Review
- Multimodularity of a GH10 Xylanase Found in the Termite Gut Metagenome.Applied and environmental microbiology · 2021Article
- Plant cell wall architecture guided design of CBM3-GH11 chimeras with enhanced xylanase activity using a tandem repeat left-handed β-3-prism scaffold.Computational and structural biotechnology journal · 2021Article
- Unique active-site and subsite features in the arabinogalactan-degrading GH43 exo-β-1,3-galactanase fromThe Journal of biological chemistry · 2020Article
- A pair of esterases from a commensal gut bacterium remove acetylations from all positions on complex β-mannans.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Crystal structure and substrate interactions of an unusual fungal non-CBM carrying GH26 endo-β-mannanase from Yunnania penicillata.Scientific reports · 2019Article
- Chitinolytic functions in actinobacteria: ecology, enzymes, and evolution.Applied microbiology and biotechnology · 2018Review
- Multiplex mutagenesis of four clustered CrRLK1L with CRISPR/Cas9 exposes their growth regulatory roles in response to metal ions.Scientific reports · 2018Article
- Unraveling the sequence of cytosolic reactions in the export of GspB adhesin fromThe Journal of biological chemistry · 2018Article
- Transcriptome and secretome analysis of Aspergillus fumigatus in the presence of sugarcane bagasse.BMC genomics · 2018Article
- Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases.Biotechnology for biofuels · 2018Article
- Report on the Current Inventory of the Toolbox for Plant Cell Wall Analysis: Proteinaceous and Small Molecular Probes.Frontiers in plant science · 2018Review
- Mechanistic Pathways for Peptidoglycan O-Acetylation and De-O-Acetylation.Frontiers in microbiology · 2018Review
Corrections and comments
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Authors and funding
21 authors at 6 institutions in 3 countries.
Funding
Abstract
Enzymes that hydrolyze complex carbohydrates play important roles in numerous biological processes that result in the maintenance of marine and terrestrial life. These enzymes often contain noncatalytic carbohydrate binding modules (CBMs) that have important substrate-targeting functions. In general, there is a tight correlation between the ligands recognized by bacterial CBMs and the substrate specificity of the appended catalytic modules. Through high-resolution structural studies, we demonstrate that the architecture of the ligand binding sites of 4 distinct family 35 CBMs (CBM35s), appended to 3 plant cell wall hydrolases and the exo-beta-D-glucosaminidase CsxA, which contributes to the detoxification and metabolism of an antibacterial fungal polysaccharide, is highly conserved and imparts specificity for glucuronic acid and/or Delta4,5-anhydrogalaturonic acid (Delta4,5-GalA). Delta4,5-GalA is released from pectin by the action of pectate lyases and as such acts as a signature molecule for plant cell wall degradation. Thus, the CBM35s appended to the 3 plant cell wall hydrolases, rather than targeting the substrates of the cognate catalytic modules, direct their appended enzymes to regions of the plant that are being actively degraded. Significantly, the CBM35 component of CsxA anchors the enzyme to the bacterial cell wall via its capacity to bind uronic acid sugars. This latter observation reveals an unusual mechanism for bacterial cell wall enzyme attachment. This report shows that the biological role of CBM35s is not dictated solely by their carbohydrate specificities but also by the context of their target ligands.
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