ArticleProceedings of the National Academy of Sciences of the United States of America2009
LapD is a bis-(3',5')-cyclic dimeric GMP-binding protein that regulates surface attachment by Pseudomonas fluorescens Pf0-1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 176 papers.
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Who cites it
176 citing papers in PubMed, 291 citations in OpenAlex.
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- Structural analyses uncover protease-adhesin interactions and c-di-GMP receptor regulation in sulfate-reducing bacteria.Nature communications · 2026Article
- Peptide-based ligand antagonists block a Vibrio cholerae adhesin.FEBS letters · 2026Article
- Regulation of indigoidine production inJournal of bacteriology · 2026Article
- Architecture and regulatory functions of c-di-GMP signaling in classical Bordetella species.FEMS microbiology reviews · 2026Review
- The dual-species biofilm formed by Staphylococcus aureus and Pseudomonas fluorescens exhibited enhanced resistance to disinfectants.NPJ science of food · 2025Article
- Dynamic Executors of Bacterial Signals: Functional Versatility and Regulatory Networks of c-di-GMP Effectors.Biomolecules · 2025Review
- Genetic and biochemical analyses reveal direct interactions between LitR and genes important forJournal of bacteriology · 2025Article
- Biofilm Dispersal inMicroorganisms · 2025Article
- A WYL domain transcription factor regulates Lactiplantibacillus plantarum intestinal colonization via perceiving c-di-GMP.Nature communications · 2025Article
- Absence of biofilm adhesin proteins changes surface attachment and cell strategy forJournal of bacteriology · 2025Article
- Tetracycline inducesApplied and environmental microbiology · 2025Article
- BpfD Is a c-di-GMP Effector Protein Playing a Key Role for Pellicle Biosynthesis inInternational journal of molecular sciences · 2024Article
- How sensor Amt-like proteins integrate ammonium signals.Science advances · 2024Article
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- Reconstitution of a biofilm adhesin system from a sulfate-reducing bacterium inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Review
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- Cellulase Promotes Mycobacterial Biofilm Dispersal in Response to a Decrease in the Bacterial Metabolite Gamma-Aminobutyric Acid.International journal of molecular sciences · 2024Article
- Role of extracellular matrix components in biofilm formation and adaptation ofFrontiers in microbiology · 2024Article
116 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The second messenger cyclic dimeric GMP (c-di-GMP) regulates surface attachment and biofilm formation by many bacteria. For Pseudomonas fluorescens Pf0-1, c-di-GMP impacts the secretion and localization of the adhesin LapA, which is absolutely required for stable surface attachment and biofilm formation by this bacterium. In this study we characterize LapD, a unique c-di-GMP effector protein that controls biofilm formation by communicating intracellular c-di-GMP levels to the membrane-localized attachment machinery via its periplasmic domain. LapD contains degenerate and enzymatically inactive diguanylate cyclase and c-di-GMP phosphodiesterase (EAL) domains and binds to c-di-GMP through a degenerate EAL domain. We present evidence that LapD utilizes an inside-out signaling mechanism: binding c-di-GMP in the cytoplasm and communicating this signal to the periplasm via its periplasmic domain. Furthermore, we show that LapD serves as the c-di-GMP receptor connecting environmental modulation of intracellular c-di-GMP levels by inorganic phosphate to regulation of LapA localization and thus surface commitment by P. fluorescens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.