ArticleProceedings of the National Academy of Sciences of the United States of America2009
Molecular basis of the interactions between the p73 N terminus and p300: effects on transactivation and modulation by phosphorylation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- Dichotomous transactivation domains contribute to growth inhibitory and promotion functions of TAp73.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- DARPins detect the formation of hetero-tetramers of p63 and p73 in epithelial tissues and in squamous cell carcinoma.Cell death & disease · 2023Article
- Structural diversity of p63 and p73 isoforms.Cell death and differentiation · 2022Review
- Review
- Targeting Post-Translational Modifications of the p73 Protein: A Promising Therapeutic Strategy for Tumors.Cancers · 2021Review
- Isoform-Specific Roles of Mutant p63 in Human Diseases.Cancers · 2021Review
- DNA Damaged Induced Cell Death in Oocytes.Molecules (Basel, Switzerland) · 2020Review
- Structural insights into TAZ2 domain-mediated CBP/p300 recruitment by transactivation domain 1 of the lymphopoietic transcription factor E2A.The Journal of biological chemistry · 2020Article
- Cytoplasmic pro-apoptotic function of the tumor suppressor p73 is mediated through a modified mode of recognition of the anti-apoptotic regulator Bcl-XThe Journal of biological chemistry · 2018Article
- PLK2 phosphorylates and inhibits enriched TAp73 in human osteosarcoma cells.Cancer medicine · 2016Article
- Structure and apoptotic function of p73.BMB reports · 2015Review
- Structural convergence of unstructured p53 family transactivation domains in MDM2 recognition.Cell cycle (Georgetown, Tex.) · 2015Article
- Polo-like kinase 2 acting as a promoter in human tumor cells with an abundance of TAp73.OncoTargets and therapy · 2015Article
- Bioinformatics study of cancer-related mutations within p53 phosphorylation site motifs.International journal of molecular sciences · 2014Article
- Pathological unfoldomics of uncontrolled chaos: intrinsically disordered proteins and human diseases.Chemical reviews · 2014Review
- p53 Family and Cellular Stress Responses in Cancer.Frontiers in oncology · 2014Review
- Alterations of p63 and p73 in human cancers.Sub-cellular biochemistry · 2014Review
- The HTLV-1-encoded protein HBZ directly inhibits the acetyl transferase activity of p300/CBP.Nucleic acids research · 2012Article
- Quasi-anharmonic analysis reveals intermediate states in the nuclear co-activator receptor binding domain ensemble.Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing · 2012Article
- p53 Isoforms: An Intracellular Microprocessor?Genes & cancer · 2011Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The transcription factor p73 belongs to the p53 family of proteins and can transactivate a number of target genes in common with p53. Here, we characterized the interaction of the p73 N terminus with four domains of the transcriptional coactivator p300 and with the negative regulator Mdm2 by using biophysical and cellular measurements. We found that, like p53, the N terminus of p73 contained two distinct transactivation subdomains, comprising residues 10-30 and residues 46-67. The p73 N terminus bound weakly to the Taz1, Kix, and IBiD domains of p300 but with submicromolar affinity for Taz2, in contrast to previous reports. We found weaker binding of the p73 N terminus to the p300 domains in vitro correlated with a significant decrease in transactivation activity in a cell line for the QS and T14A mutants, and tighter binding of the phosphomimetic T14D in vitro correlated with an increase in vivo. Further, we found that phosphorylation of T14 increased the affinity of the p73 N terminus for Taz2 10-fold. The phosphomimetic p73alpha T14D caused increased levels of transactivation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.