ArticleApplied and environmental microbiology2009
Variation in bacterial ATP level and proton motive force due to adhesion to a solid surface.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
- Exploring the functionality of mesoporous silica nanoparticles as a prebiotic agent.Antonie van Leeuwenhoek · 2025Article
- An Addendum to the Chemiosmotic Theory of Mitochondrial Activity: The Role of RNA as a Proton Sink.Biomolecules · 2025Review
- Influence of Dead Cells Killed by Industrial Biocides (BAC and DBNPA) on Biofilm Formation.Antibiotics (Basel, Switzerland) · 2024Article
- Small-Molecule Analysis Based on DNA Strand Displacement Using a Bacteriorhodopsin Photoelectric Transducer: Taking ATP as an Example.Sensors (Basel, Switzerland) · 2023Article
- Motility Increases the Numbers and Durations of Cell-Surface Engagements forACS applied materials & interfaces · 2022Article
- Novel Thiadiazole-Based Molecules as Promising Inhibitors of Black Fungi and Pathogenic Bacteria: In Vitro Antimicrobial Evaluation and Molecular Docking Studies.Molecules (Basel, Switzerland) · 2022Article
- Hibiscus Acid fromMolecules (Basel, Switzerland) · 2022Article
- Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis.Scientific reports · 2021Article
- Acid tolerance in early colonizers of oral biofilms.BMC microbiology · 2021Article
- Optimisation of bacterial release from a stable microfluidic-generated water-in-oil-in-water emulsion.RSC advances · 2021Article
- Metabolism, ATP production and biofilm generation by Staphylococcus epidermidis in either respiratory or fermentative conditions.AMB Express · 2020Article
- How bacteria recognise and respond to surface contact.FEMS microbiology reviews · 2020Review
- RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes.Pathogens (Basel, Switzerland) · 2016Article
- Influences of Adhesion Variability on the "Living" Dynamics of Filamentous Bacteria in Microfluidic Channels.Molecules (Basel, Switzerland) · 2016Article
- Metabolic Responses of Bacterial Cells to Immobilization.Molecules (Basel, Switzerland) · 2016Review
- Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments-a Review.Frontiers in microbiology · 2016Review
- Aminoglycoside inhibition of Staphylococcus aureus biofilm formation is nutrient dependent.Journal of medical microbiology · 2014Article
- Article
- Bacteria-surface interactions.Soft matter · 2013Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial adhesion to natural and man-made surfaces can be beneficial or detrimental, depending on the system at hand. Of vital importance is how the process of adhesion affects the bacterial metabolic activity. If activity is enhanced, this may help the cells colonize the surface, whereas if activity is reduced, it may inhibit colonization. Here, we report a study demonstrating that adhesion of both Escherichia coli and Bacillus brevis onto a glass surface resulted in enhanced metabolic activity, assessed through ATP measurements. Specifically, ATP levels were found to increase two to five times upon adhesion compared to ATP levels in corresponding planktonic cells. To explain this effect on ATP levels, we propose the hypothesis that bacteria can take advantage of a link between cellular bioenergetics (proton motive force and ATP formation) and the physiochemical charge regulation effect, which occurs as a surface containing ionizable functional groups (e.g., the bacterial cell surface) approaches another surface. As the bacterium approaches the surface, the charge regulation effect causes the charge and pH at the cell surface to vary as a function of separation distance. With negatively charged surfaces, this results in a decrease in pH at the cell surface, which enhances the proton motive force and ATP concentration. Calculations demonstrated that a change in pH across the cell membrane of only 0.2 to 0.5 units is sufficient to achieve the observed ATP increases. Similarly, the hypothesis indicates that positively charged surfaces will decrease metabolic activity, and results from studies of positively charged surfaces support this finding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.