ArticleThe Plant cell2009
A novel fatty Acyl-CoA Synthetase is required for pollen development and sporopollenin biosynthesis in Arabidopsis.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 148 papers.
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Who cites it
148 citing papers in PubMed, 277 citations in OpenAlex.
- Combined Analysis of Metabolome and Transcriptome RevealsMetabolites · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Anther middle layer fatty acid synthesis is essential for pollen development and fertility restoration at low temperature in Arabidopsis.Plant physiology · 2026Article
- Comparative Study on Cytological Characteristics of Reproductive Organs and Embryonic Development in Three Forms of the Mycoheterotrophic OrchidPlants (Basel, Switzerland) · 2026Article
- Aqueous-Phase Polycondensation of Hydroxy Fatty Acids via a Whole-Cell CoA Activation-Acyltransferase Cascade.JACS Au · 2026Article
- Identification of phenological QTLs using a combination of high- and low-coverage whole genome sequencing in Japanese plum (Horticulture research · 2026Article
- Bee-mediated pollination enhances fruit set and seed yield inHorticulture research · 2025Article
- Article
- Insights into the Molecular Basis of Pollen Coat Development and Its Role in Male Sterility.International journal of molecular sciences · 2025Review
- Comparative proteomic analysis of self-compatible and self-incompatible genotypes of fennel (Foeniculum vulgare Mill.).BMC plant biology · 2025Article
- Genetic Analysis and Fine Mapping of Spontaneously Mutated Male Sterility Gene in Chinese Cabbage (Plants (Basel, Switzerland) · 2025Article
- The dynamic regulatory network of stamens and pistils in papaya.BMC plant biology · 2025Article
- OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice.Rice (New York, N.Y.) · 2024Article
- Article
- The transcription factors and pathways underpinning male reproductive development in Arabidopsis.Frontiers in plant science · 2024Review
- Overexpression ofFrontiers in plant science · 2024Article
- Single-Cell Transcriptome Atlas and Regulatory Dynamics in Developing Cotton Anthers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- CRISPR/Cas9-mediated disruption of CjACOS5 confers no-pollen formation on sugi trees (Cryptomeria japonica D. Don).Scientific reports · 2023Article
- Proteome Analysis ofPlants (Basel, Switzerland) · 2023Article
- Transcriptomic and Proteomic Analyses of Celery Cytoplasmic Male Sterile Line and Its Maintainer Line.International journal of molecular sciences · 2023Article
88 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acyl-CoA Synthetase (ACOS) genes are related to 4-coumarate:CoA ligase (4CL) but have distinct functions. The Arabidopsis thaliana ACOS5 protein is in clade A of Arabidopsis ACOS proteins, the clade most closely related to 4CL proteins. This clade contains putative nonperoxisomal ACOS enzymes conserved in several angiosperm lineages and in the moss Physcomitrella patens. Although its function is unknown, ACOS5 is preferentially expressed in the flowers of all angiosperms examined. Here, we show that an acos5 mutant produced no pollen in mature anthers and no seeds by self-fertilization and was severely compromised in pollen wall formation apparently lacking sporopollenin or exine. The phenotype was first evident at stage 8 of anther development and correlated with maximum ACOS5 mRNA accumulation in tapetal cells at stages 7 to 8. Green fluorescent protein-ACOS5 fusions showed that ACOS5 is located in the cytoplasm. Recombinant ACOS5 enzyme was active against oleic acid, allowing kinetic constants for ACOS5 substrates to be established. Substrate competition assays indicated broad in vitro preference of the enzyme for medium-chain fatty acids. We propose that ACOS5 encodes an enzyme that participates in a conserved and ancient biochemical pathway required for sporopollenin monomer biosynthesis that may also include the Arabidopsis CYP703A2 and MS2 enzymes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.