ArticleJournal of bacteriology2009
Nanoscale structural and mechanical properties of nontypeable Haemophilus influenzae biofilms.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
- Nanomechanical characterization of soft nanomaterial using atomic force microscopy.Materials today. Bio · 2025Review
- Extracellular Polymeric Substance Protects Some Cells in anMicroorganisms · 2021Article
- Nanomotion Detection-Based Rapid Antibiotic Susceptibility Testing.Antibiotics (Basel, Switzerland) · 2021Review
- A Refined View of Airway Microbiome in Chronic Obstructive Pulmonary Disease at Species and Strain-Levels.Frontiers in microbiology · 2020Article
- A culture-dependent and metagenomic approach of household drinking water from the source to point of use in a developing country.Water research X · 2019Article
- In vivo detection of nanometer-scale structural changes of the human tympanic membrane in otitis media.Scientific reports · 2018Article
- Insights on persistent airway infection by non-typeable Haemophilus influenzae in chronic obstructive pulmonary disease.Pathogens and disease · 2017Review
- Contour and persistence length of Corynebacterium diphtheriae pili by atomic force microscopy.European biophysics journal : EBJ · 2012Article
- Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy.Applied microbiology and biotechnology · 2011Article
- A network model for biofilm development in Escherichia coli K-12.Theoretical biology & medical modelling · 2011Article
- Extracellular matrix formation enhances the ability of Streptococcus pneumoniae to cause invasive disease.PloS one · 2011Article
- A tactile response in Staphylococcus aureus.Biophysical journal · 2010Article
- Variations in the Nanomechanical Properties of Virulent and Avirulent Listeria monocytogenes.Soft matter · 2010Article
- Differential lipopolysaccharide core capping leads to quantitative and correlated modifications of mechanical and structural properties in Pseudomonas aeruginosa biofilms.Journal of bacteriology · 2009Article
- Potential role of atomic force microscopy in systems biology.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Nontypeable Haemophilus influenzae (NTHI) bacteria are commensals in the human nasopharynx, as well as pathogens associated with a spectrum of acute and chronic infections. Two important factors that influence NTHI pathogenicity are their ability to adhere to human tissue and their ability to form biofilms. Extracellular polymeric substances (EPS) and bacterial appendages such as pili critically influence cell adhesion and intercellular cohesion during biofilm formation. Structural components in the outer cell membrane, such as lipopolysaccharides, also play a fundamental role in infection of the host organism. In spite of their importance, these pathogenic factors are not yet well characterized at the nanoscale. Here, atomic force microscopy (AFM) was used in aqueous environments to visualize structural details, including probable Hif-type pili, of live NTHI bacteria at the early stages of biofilm formation. Using single-molecule AFM-based spectroscopy, the molecular elasticities of lipooligosaccharides present on NTHI cell surfaces were analyzed and compared between two strains (PittEE and PittGG) with very different pathogenicity profiles. Furthermore, the stiffness of single cells of both strains was measured and subsequently their turgor pressure was estimated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.