ArticleBiophysical journal2009
Cardiac myocyte excitation by ultrashort high-field pulses.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 79 citations in OpenAlex.
- Precise mimicry of physiological CaBioengineering & translational medicine · 2026Article
- Protein-mediated electroporation: Pore formation in a calcium voltage-sensing domain induced by positive and negative nanosecond pulsed electric fields.Protein science : a publication of the Protein Society · 2025Article
- Excitation and electroporation in genetically engineered excitable S-HEK cells exposed to electric pulses of different durations.Scientific reports · 2025Article
- Genetically engineered HEK cells as a valuable tool for studying electroporation in excitable cells.Scientific reports · 2024Article
- Pulsed Field Ablation for the Treatment of Atrial Fibrillation: A Review and a Look into its Future.The heart surgery forum · 2024Article
- Protein-Mediated Electroporation in a Cardiac Voltage-Sensing Domain Due to an nsPEF Stimulus.International journal of molecular sciences · 2023Article
- Article
- Nanosecond pulsed electric field (nsPEF) and vaccines: a novel technique for the inactivation of SARS-CoV-2 and other viruses?Annals of medicine · 2022Article
- Nanosecond Pulsed Electric Field (nsPEF): Opening the Biotechnological Pandora's Box.International journal of molecular sciences · 2022Review
- Electroporation and cell killing by milli- to nanosecond pulses and avoiding neuromuscular stimulation in cancer ablation.Scientific reports · 2022Article
- Electrical Stimulation and Cellular Behaviors in Electric Field in Biomedical Research.Materials (Basel, Switzerland) · 2021Review
- Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro.Molecules (Basel, Switzerland) · 2021Article
- Exploring the Conformational Changes Induced by Nanosecond Pulsed Electric Fields on the Voltage Sensing Domain of a CaMembranes · 2021Article
- The interplay of excitation and electroporation in nanosecond pulse stimulation.Bioelectrochemistry (Amsterdam, Netherlands) · 2020Review
- Pulsed Electric Fields Can Create Pores in the Voltage Sensors of Voltage-Gated Ion Channels.Biophysical journal · 2020Article
- Using Nanosecond Shocks for Cardiac Defibrillation.Bioelectricity · 2019Review
- Excitation and electroporation by MHz bursts of nanosecond stimuli.Biochemical and biophysical research communications · 2019Article
- Nanosecond pulsed electric fields induce extracellular release of chromosomal DNA and histone citrullination in neutrophil-differentiated HL-60 cells.Scientific reports · 2019Article
- Ultrashort nanosecond electric pulses evoke heterogeneous patterns of CaBiochimica et biophysica acta. Biomembranes · 2019Article
- Excitation of murine cardiac myocytes by nanosecond pulsed electric field.Journal of cardiovascular electrophysiology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
In unexcitable, noncardiac cells, ultrashort (nanosecond) high-voltage (megavolt-per-meter) pulsed electrical fields (nsPEF) can mobilize intracellular Ca2+ and create transient nanopores in the plasmalemma. We studied Ca2+ responses to nsPEF in cardiac cells. Fluorescent Ca2+ or voltage signals were recorded from isolated adult rat ventricular myocytes deposited in an electrode microchamber and stimulated with conventional pulses (CPs; 0.5-2.4 kV/cm, 1 ms) or nsPEF (10-80 kV/cm, 4 ns). nsPEF induced Ca2+ transients in 68/104 cells. Repeating nsPEF increased the likelihood of Ca2+ transient induction (61.8% for <10 nsPEF vs. 80.6% for > or =10 nsPEF). Repetitive Ca2+ waves arising at the anodal side and Ca2+ destabilization occurred after repeated nsPEF (12/29) or during steady-state single nsPEF delivery at 2 Hz. Removing extracellular Ca2+ abolished responses to nsPEF. Verapamil did not affect nsPEF-induced Ca2+ transients, but decreased responses to CP. Tetrodotoxin and KB-R7943 increased the repetition threshold in response to nsPEF: 1-20 nsPEF caused local anodal Ca2+ waves without Ca2+ transients, and > or =20 nsPEF caused normal transients. Ryanodine-thapsigargin and caffeine protected against nsPEF-induced Ca2+ waves and showed less recovery of diastolic Ca2+ levels than CP. Voltage recordings demonstrated action potentials triggered by nsPEF, even in the presence of tetrodotoxin. nsPEF can mobilize intracellular Ca2+ in cardiac myocytes by inducing action potentials. Anodal Ca2+ waves and resistance to Na+ and Ca2+ channel blockade suggest nonselective ion channel transport via sarcolemmal nanopores as a triggering mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.