ArticleBiophysical journal2009
Impact of actin rearrangement and degranulation on the membrane structure of primary mast cells: a combined atomic force and laser scanning confocal microscopy investigation.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 70 citations in OpenAlex.
- Measurement of anti-allergic effects using flow-through piezoelectric biosensors based on the mast cells exocytosis.Mikrochimica acta · 2026Article
- Multistep IgE Mast Cell Desensitization Is a Dose- and Time-Dependent Process Partially Regulated by SHIP-1.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- The Indigenous Volatile Inhibitor 2-Methyl-2-butene Impacts Biofilm Formation and Interspecies Interaction of the Pathogenic Mucorale Rhizopus arrhizus.Microbial ecology · 2022Article
- RACK1 plays a critical role in mast cell secretion and Ca2+ mobilization by modulating F-actin dynamics.Journal of cell science · 2021Article
- Cytoskeletal Transport, Reorganization, and Fusion Regulation in Mast Cell-Stimulus Secretion Coupling.Frontiers in cell and developmental biology · 2021Review
- Direct Observations of Silver Nanowire-Induced Frustrated Phagocytosis among NR8383 Lung Alveolar Macrophages.The journal of physical chemistry. B · 2020Article
- Cell Type- and Stimulation-Dependent Transcriptional Programs Regulated by Atg16L1 and Its Crohn's Disease Risk Variant T300A.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Aurora kinase inhibitor tozasertib suppresses mast cell activation in vitro and in vivo.British journal of pharmacology · 2020Article
- Anaphylactic Degranulation of Mast Cells: Focus on Compound Exocytosis.Journal of immunology research · 2019Review
- Applying Pattern Recognition to High-Resolution Images to Determine Cellular Signaling Status.IEEE transactions on nanobioscience · 2017Article
- TRPM4-mediated control of FcεRI-evoked Ca(2+) elevation comprises enhanced plasmalemmal trafficking of TRPM4 channels in connective tissue type mast cells.Scientific reports · 2016Article
- Article
- Mechanism of mucosal permeability enhancement of CriticalSorb® (Solutol® HS15) investigated in vitro in cell cultures.Pharmaceutical research · 2015Article
- Rictor negatively regulates high-affinity receptors for IgE-induced mast cell degranulation.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Characterization of mast cell secretory granules and their cell biology.DNA and cell biology · 2014Article
- Mast cell secretory granules: armed for battle.Nature reviews. Immunology · 2014Review
- Spatiotemporally and mechanically controlled triggering of mast cells using atomic force microscopy.Immunologic research · 2014Article
- Regulation of cellular communication by signaling microdomains in the blood vessel wall.Pharmacological reviews · 2014Review
- Prevention of F-actin assembly switches the response to SCF from chemotaxis to degranulation in human mast cells.European journal of immunology · 2013Article
- Receptor-mediated transcytosis: a mechanism for active extravascular transport of nanoparticles in solid tumors.Journal of controlled release : official journal of the Controlled Release Society · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Degranulation of bone marrow-derived mast cells (BMMCs) triggered by antigens (e.g., 2,4-dinitrophenylated bovine serum albumin (DNP-BSA) and secretagogues (e.g., poly-L-lysine) was investigated by combined atomic force microscopy (AFM) and laser scanning confocal microscopy (LSCM). This combination enables the simultaneous visualization and correlation of membrane morphology with cytoskeletal actin arrangement and intracellular granules. Two degranulation mechanisms and detailed membrane structures that directly corresponded to the two stimuli were revealed. In DNP-BSA triggered activation, characteristic membrane ridges formed in accordance with the rearrangement of underlying F-actin networks. Individual granules were visualized after they released their contents, indicating a "kiss-and-run" pathway. In BMMCs stimulated by poly-L-lysine, lamellopodia and filopodia were observed in association with the F-actin assemblies at and near the cell periphery, whereas craters were observed on the central membrane lacking F-actin. These craters represent a new membrane feature resulting from the "kiss-and-merge" granule fusion. This work provides what we believe is important new insight into the local membrane structures in correlation with the cytoskeleton arrangement and detailed degranulation processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.