ArticleBiophysical journal2009
Buffer interference with protein dynamics: a case study on human liver fatty acid binding protein.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Effects of ligand binding on dynamics of fatty acid binding protein and interactions with membranes.Biophysical journal · 2022Article
- Repurposing drugs for treatment of SARS-CoV-2 infection: computational design insights into mechanisms of action.Journal of biomolecular structure & dynamics · 2022Article
- Conformational exchange of fatty acid binding protein induced by protein-nanodisc interactions.Biophysical journal · 2021Article
- Interference of pH buffer with PbMetallomics : integrated biometal science · 2020Article
- Atomistic Insights into the Functional Instability of the Second Helix of Fatty Acid Binding Protein.Biophysical journal · 2019Article
- Addressing a Common Misconception: Ammonium Acetate as Neutral pH "Buffer" for Native Electrospray Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- MOPS and coxsackievirus B3 stability.Virology · 2017Article
- Entire-Dataset Analysis of NMR Fast-Exchange Titration Spectra: A MgThe journal of physical chemistry. B · 2016Article
- Review
- Universal buffers for use in biochemistry and biophysical experiments.AIMS biophysics · 2015Article
- The human liver fatty acid binding protein T94A variant alters the structure, stability, and interaction with fibrates.Biochemistry · 2013Article
- Inhibitors of Fatty Acid Synthesis Induce PPAR α -Regulated Fatty Acid β -Oxidative Genes: Synergistic Roles of L-FABP and Glucose.PPAR research · 2013Article
- Solution structure and backbone dynamics of human liver fatty acid binding protein: fatty acid binding revisited.Biophysical journal · 2012Article
- Mapping site-specific changes that affect stability of the N-terminal domain of calmodulin.Molecular pharmaceutics · 2012Article
- Millisecond timescale dynamics of human liver fatty acid binding protein: testing of its relevance to the ligand entry process.Biophysical journal · 2010Article
- Protein-Inhibitor Interaction Studies Using NMR.Applications of NMR spectroscopyArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Selection of suitable buffer types is often a crucial step for generating appropriate protein samples for NMR and x-ray crystallographic studies. Although the possible interaction between MES buffer (2-(N-morpholino)ethanesulfonic acid) and proteins has been discussed previously, the interaction is usually thought to have no significant effects on the structures of proteins. In this study, we demonstrate the direct, albeit weak, interaction between MES and human liver fatty acid binding protein (hLFABP). Rather than affecting the structure of hLFABP, we found that the dynamics of hLFABP, which were previously proposed to be relevant to its functions, were significantly affected by the binding of hLFABP with MES. Buffer interference with protein dynamics was also demonstrated with Bis-Tris buffer, which is quite different from MES and fatty acids in terms of their molecular structures and properties. This result, to our knowledge, is the first published report on buffer interference with protein dynamics on a microsecond to millisecond timescale and could represent a generic problem in the studies of functionally relevant protein dynamics. Although being a fortuity, our finding of buffer-induced changes in protein dynamics offers a clue to how hLFABP accommodates its ligands.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.