ArticleBiophysical journal2009
The photocycle and proton translocation pathway in a cyanobacterial ion-pumping rhodopsin.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 116 citations in OpenAlex.
- Rhobot-Screen: an integrated robotic platform for functional screening of rhodopsin variants.BMC biology · 2026Article
- Carotenoids bind rhodopsins and act as photocycle-accelerating pigments in marine Bacteroidota.Nature microbiology · 2025Article
- Proteorhodopsin insights into the molecular mechanism of vectorial proton transport.Science advances · 2025Article
- Driving forces of proton-pumping rhodopsins.Biophysical journal · 2024Article
- Engineering bionanoreactor in bacteria for efficient hydrogen production.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Cyanorhodopsin-II represents a yellow-absorbing proton-pumping rhodopsin clade within cyanobacteria.The ISME journal · 2024Article
- Mutagenic analysis of the bundle-shaped phycobilisome from Gloeobacter violaceus.Photosynthesis research · 2023Article
- Impact of protein-chromophore interaction on the retinal excited state and photocycle ofChemical science · 2023Article
- Unlocking chloride sensing in the red at physiological pH with a fluorescent rhodopsin-based host.Chemical communications (Cambridge, England) · 2023Article
- Retinal-Carotenoid Interactions in a Sodium-Ion-Pumping Rhodopsin: Implications on Oligomerization and Thermal Stability.The journal of physical chemistry. B · 2023Article
- Light-driven Proton Pumps as a Potential Regulator for Carbon Fixation in Marine Diatoms.Microbes and environments · 2023Article
- Coupling a Live Cell Directed Evolution Assay with Coevolutionary Landscapes to Engineer an Improved Fluorescent Rhodopsin Chloride Sensor.ACS synthetic biology · 2022Article
- Structural characterization of proton-pumping rhodopsin lacking a cytoplasmic proton donor residue by X-ray crystallography.The Journal of biological chemistry · 2022Article
- A single point mutation converts a proton-pumping rhodopsin into a red-shifted, turn-on fluorescent sensor for chloride.Chemical science · 2021Article
- Assembly of Natively Synthesized Dual Chromophores Into Functional Actinorhodopsin.Frontiers in microbiology · 2021Article
- Applicability of Styrene-Maleic Acid Copolymer for Two Microbial Rhodopsins, RxR and HsSRI.Biophysical journal · 2020Article
- A unique clade of light-driven proton-pumping rhodopsins evolved in the cyanobacterial lineage.Scientific reports · 2020Article
- The chirality origin of retinal-carotenoid complex in gloeobacter rhodopsin: a temperature-dependent excitonic coupling.Scientific reports · 2020Article
- Effect of a bound anion on the structure and dynamics of halorhodopsin fromStructural dynamics (Melville, N.Y.) · 2019Article
- X-ray Crystallographic Structure and Oligomerization of Gloeobacter Rhodopsin.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of thylakoidless cyanobacterium Gloeobacter violaceus encodes a fast-cycling rhodopsin capable of light-driven proton transport. We characterize the dark state, the photocycle, and the proton translocation pathway of GR spectroscopically. The dark state of GR contains predominantly all-trans-retinal and, similar to proteorhodopsin, does not show the light/dark adaptation. We found an unusually strong coupling between the conformation of the retinal and the site of Glu132, the homolog of Asp96 of BR. Although the photocycle of GR is similar to that of proteorhodopsin in general, it differs in accumulating two intermediates typical for BR, the L-like and the N-like states. The latter state has a deprotonated cytoplasmic proton donor and is spectrally distinct from the strongly red-shifted N intermediate known for proteorhodopsin. The proton uptake precedes the release and occurs during the transition to the O intermediate. The proton translocation pathway of GR is similar to those of other proton-pumping rhodopsins, involving homologs of BR Schiff base proton acceptor and donor Asp85 and Asp96 (Asp121 and Glu132). We assigned a pair of FTIR bands (positive at 1749 cm(-1) and negative at 1734 cm(-1)) to the protonation and deprotonation, respectively, of these carboxylic acids.
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