ArticleThrombosis research2009
Ultrasound-enhanced thrombolysis with tPA-loaded echogenic liposomes.
Article in Thrombosis research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 127 citations in OpenAlex.
- Ultrasound-Mediated Thrombolysis: From Mechanistic Insights to Advanced Nanoplatforms and Clinical Translation.Advanced healthcare materials · 2026Review
- Emerging Targets, Novel Directions, and Innovative Approaches in Thrombosis Therapy.Aging and disease · 2025Review
- Overview of Venous Thromboembolism and Emerging Therapeutic Technologies Based on Nanocarriers-Mediated Drug Delivery Systems.Molecules (Basel, Switzerland) · 2024Review
- Targeted nano-delivery strategies for facilitating thrombolysis treatment in ischemic stroke.Drug delivery · 2021Article
- Thrombolytic Agents: Nanocarriers in Controlled Release.Small (Weinheim an der Bergstrasse, Germany) · 2020Review
- Vascular Nanomedicine: Current Status, Opportunities, and Challenges.Seminars in thrombosis and hemostasis · 2020Review
- Advances in Sonothrombolysis Techniques Using Piezoelectric Transducers.Sensors (Basel, Switzerland) · 2020Review
- Engineered microparticles and nanoparticles for fibrinolysis.Journal of thrombosis and haemostasis : JTH · 2019Review
- In vitro characterization of sonothrombolysis and echocontrast agents to treat ischemic stroke.Scientific reports · 2019Article
- An unmet clinical need: The history of thrombus imaging.Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology · 2019Review
- For Whom the Bubble Grows: Physical Principles of Bubble Nucleation and Dynamics in Histotripsy Ultrasound Therapy.Ultrasound in medicine & biology · 2019Review
- Recent strategies on targeted delivery of thrombolytics.Asian journal of pharmaceutical sciences · 2019Review
- Tissue plasminogen activator-based nanothrombolysis for ischemic stroke.Expert opinion on drug delivery · 2018Article
- Spectrophotometric analysis of thrombolytic activity: SATA assay.BioImpacts : BI · 2018Article
- Translational initiatives in thrombolytic therapy.Frontiers of medicine · 2017Review
- In vitro thrombolytic efficacy of echogenic liposomes loaded with tissue plasminogen activator and octafluoropropane gas.Physics in medicine and biology · 2017Article
- Article
- Effect of Thrombus Composition and Viscosity on Sonoreperfusion Efficacy in a Model of Micro-Vascular Obstruction.Ultrasound in medicine & biology · 2016Article
- Efficacy of histotripsy combined with rt-PA in vitro.Physics in medicine and biology · 2016Article
- Microfluidic manufacture of rt-PA -loaded echogenic liposomes.Biomedical microdevices · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
background and purposeCurrently, the only FDA-approved therapy for acute ischemic stroke is the administration of recombinant tissue plasminogen activator (tPA). Echogenic liposomes (ELIP), phospholipid vesicles filled with gas and fluid, can be manufactured to incorporate tPA. Also, transcranial ultrasound-enhanced thrombolysis can increase the recanalization rate in stroke patients. However, there is little data on lytic efficacy of combining ultrasound, echogenic liposomes, and tPA treatment. In this study, we measure the effects of pulsed 120-kHz ultrasound on the lytic efficacy of tPA and tPA-incorporating ELIP (t-ELIP) in an in-vitro human clot model. It is hypothesized that t-ELIP exhibits similar lytic efficacy to that of rt-PA.
methodsBlood was drawn from 22 subjects after IRB approval. Clots were made in 20-microL pipettes, and placed in a water tank for microscopic visualization during ultrasound and drug treatment. Clots were exposed to combinations of [tPA]=3.15 microg/ml, [t-ELIP]=3.15 microg/ml, and 120-kHz ultrasound for 30 minutes at 37 degrees C in human plasma. At least 12 clots were used for each treatment. Clot lysis over time was imaged and clot diameter was measured over time, using previously developed imaging analysis algorithms. The fractional clot loss (FCL), which is the decrease in mean clot width at the end of lytic treatment, was used as a measure of lytic efficacy for the various treatment regimens.
resultsThe fractional clot loss FCL was 31% (95% CI: 26-37%) and 71% (56-86%) for clots exposed to tPA alone or tPA with 120 kHz ultrasound. Similarly, FCL was 48% (31-64%) and 89% (76-100%) for clots exposed to t-ELIP without or with ultrasound.
conclusionsThe lytic efficacy of tPA containing echogenic liposomes is comparable to that of tPA alone. The addition of 120 kHz ultrasound significantly enhanced lytic treatment efficacy for both tPA and t-ELIP. Liposomes loaded with tPA may be a useful adjunct in lytic treatment with tPA.
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