ArticleNeuron2009
Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging.
Article in Neuron, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
96 citing papers in PubMed, 155 citations in OpenAlex.
- NSF is required for diverse endocytic modes by promoting fusion and fission pore closure in secretory cells.iScience · 2026Article
- Common Mechanism Underlying Synaptic Dysfunction Caused by Preformed Fibril-Induced Accumulation of α-Synuclein or Tau in a Culture Propagation Model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Post-synaptic density proteins in oligodendrocytes are required for activity-dependent myelin sheath growth.iScience · 2025Article
- Synaptic Vesicle Recycling Through the Lens of Ultrafast Endocytosis.Annual review of neuroscience · 2025Review
- CaCell reports · 2025Article
- Article
- Axonal neurotransmitter release in the regulation of myelination.Bioscience reports · 2024Review
- Article
- Clathrin mediates membrane fission and budding by constricting membrane pores.Cell discovery · 2024Article
- The CHD family chromatin remodeling enzyme, Kismet, promotes both clathrin-mediated and activity-dependent bulk endocytosis.PloS one · 2024Article
- Synaptotagmin-11 Inhibits Synaptic Vesicle Endocytosis via Endophilin A1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Novel genetically encoded tools for imaging or silencing neuropeptide release from presynaptic terminalsbioRxiv : the preprint server for biology · 2023Article
- pOpsicle: An all-optical reporter system for synaptic vesicle recycling combining pH-sensitive fluorescent proteins with optogenetic manipulation of neuronal activity.Frontiers in cellular neuroscience · 2023Article
- CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes.Nature communications · 2022Article
- Gain-of-Function Dynamin-2 Mutations Linked to Centronuclear Myopathy Impair CaInternational journal of molecular sciences · 2022Article
- Dynamin 1 controls vesicle size and endocytosis at hippocampal synapses.Cell calcium · 2022Article
- Effects of the clathrin inhibitor Pitstop-2 on synaptic vesicle recycling at a central synapseFrontiers in synaptic neuroscience · 2022Article
- Minibrain kinase and calcineurin coordinate activity-dependent bulk endocytosis through synaptojanin.The Journal of cell biology · 2021Article
- Selective inhibition of gamma aminobutyric acid release from mouse hippocampal interneurone subtypes by the volatile anaesthetic isoflurane.British journal of anaesthesia · 2021Article
- Myelination induces axonal hotspots of synaptic vesicle fusion that promote sheath growth.Current biology : CB · 2021Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Synaptic vesicle recycling is essential for maintaining efficient synaptic transmission. Detailed dissection of single-vesicle recycling still remains a major challenge. We have developed a fluorescent pH reporter that permits us to follow the fate of individual vesicles at hippocampal synapses after exocytosis. Here we show that, during low-frequency stimulation, single-vesicle fusion leads to two distinct vesicle internalizations, instead of one, as in general perception: one by a fast endocytosis pathway ( approximately 3 s), the other by a slow endocytosis pathway (after 10 s). The exocytosed vesicular proteins are preferentially recaptured in both pathways. RNAi knockdown of clathrin inhibits both pathways. As stimulation frequency increases, the number of endocytosed vesicles begins to match antecedent exocytosis. Meanwhile, the slow endocytosis is accelerated and becomes the predominant pathway. These results reveal that two pathways of endocytosis are orchestrated during neuronal activity, establishing a highly efficient endocytosis at central synapses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.