ArticleGenome biology2009
PDGF/VEGF signaling controls cell size in Drosophila.
Article in Genome biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 38 citations in OpenAlex.
- Pvr and Pvf2 Are Essential for Valve Cell Differentiation in the Larval Drosophila Heart.Genesis (New York, N.Y. : 2000) · 2026Article
- Pvf/Pvr signaling relieves white spot syndrome virus-induced lipid consumption to inhibit viral infection in shrimp.Journal of virology · 2026Article
- The RASA2/RASA3 ortholog RasGAP1 modulates obesity-linked phenotypes and is associated with leptin-analog signaling inFrontiers in endocrinology · 2026Article
- Vostok: A looping factor for the organization of the regulatory genome in the Drosophila brain.Molecular cell · 2025Article
- Pvr regulates cyst stem cell division in the Drosophila testis niche, and has functions distinct from Egfr.Cells & development · 2023Article
- Variation of TNF modulates cellular immunity of gregarious and solitary locusts against fungal pathogenProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cytokine signaling throughProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Reconstitution of Torso signaling in cultured cells suggests a role for both Trunk and Torso-like in receptor activation.Development (Cambridge, England) · 2017Article
- Sevoflurane protects rat brain endothelial barrier structure and function after hypoxia-reoxygenation injury.PloS one · 2017Article
- Control of vein network topology by auxin transport.BMC biology · 2015Article
- The effect of Beauveria bassiana infection on cell mediated and humoral immune response in house fly, Musca domestica L.Environmental science and pollution research international · 2015Article
- A systems-level interrogation identifies regulators of Drosophila blood cell number and survival.PLoS genetics · 2015Article
- Pvr expression regulators in equilibrium signal control and maintenance of Drosophila blood progenitors.eLife · 2014Article
- Switching between humoral and cellular immune responses in Drosophila is guided by the cytokine GBP.Nature communications · 2014Article
- Identification of transcriptional and metabolic programs related to mammalian cell size.Current biology : CB · 2014Article
- A functional screen reveals an extensive layer of transcriptional and splicing control underlying RAS/MAPK signaling in Drosophila.PLoS biology · 2014Article
- The Drosophila platelet-derived growth factor and vascular endothelial growth factor-receptor related (Pvr) protein ligands Pvf2 and Pvf3 control hemocyte viability and invasive migration.The Journal of biological chemistry · 2013Article
- Autocrine platelet-derived growth factor-vascular endothelial growth factor receptor-related (Pvr) pathway activity controls intestinal stem cell proliferation in the adult Drosophila midgut.The Journal of biological chemistry · 2012Article
- Article
- Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype.The Journal of cell biology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundIn multicellular animals, cell size is controlled by a limited set of conserved intracellular signaling pathways, which when deregulated contribute to tumorigenesis by enabling cells to grow outside their usual niche. To delineate the pathways controlling this process, we screened a genome-scale, image-based Drosophila RNA interference dataset for double-stranded RNAs that reduce the average size of adherent S2R+ cells.
resultsAutomated analysis of images from this RNA interference screen identified the receptor tyrosine kinase Pvr, Ras pathway components and several novel genes as regulators of cell size. Significantly, Pvr/Ras signaling also affected the size of other Drosophila cell lines and of larval hemocytes. A detailed genetic analysis of this growth signaling pathway revealed a role for redundant secreted ligands, Pvf2 and Pvf3, in the establishment of an autocrine growth signaling loop. Downstream of Ras1, growth signaling was found to depend on parallel mitogen-activated protein kinase (MAPK) and phospho-inositide-3-kinase (PI3K) signaling modules, as well as the Tor pathway.
conclusionsThis automated genome-wide screen identifies autocrine Pvf/Pvr signaling, upstream of Ras, MAPK and PI3K, as rate-limiting for the growth of immortalized fly cells in culture. Since, Pvf2/3 and Pvr show mutually exclusive in vivo patterns of gene expression, these data suggest that co-expression of this receptor-ligand pair plays a key role in driving cell autonomous growth during the establishment of Drosophila cell lines, as has been suggested to occur during tumor development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.