ArticleCellular microbiology2009
Non-invasive imaging of mouse hepatitis coronavirus infection reveals determinants of viral replication and spread in vivo.
Article in Cellular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Development of HiBiT-Tagged Recombinant Infectious Bronchitis Coronavirus for EfficientFrontiers in microbiology · 2020Article
- Non-invasive bioluminescence imaging of HCoV-OC43 infection and therapy in the central nervous system of live mice.Antiviral research · 2020Article
- Luciferase-tagged wild-type and tropism-deficient mouse cytomegaloviruses reveal early dynamics of host colonization following peripheral challenge.The Journal of general virology · 2016Article
- Fiat Luc: Bioluminescence Imaging Reveals In Vivo Viral Replication Dynamics.PLoS pathogens · 2015Article
- A review of genetic methods and models for analysis of coronavirus-induced severe pneumonitis.The Journal of general virology · 2015Review
- Visualizing the replication of respiratory syncytial virus in cells and in living mice.Nature communications · 2014Article
- In vivo imaging in NHP models of malaria: challenges, progress and outlooks.Parasitology international · 2014Review
- In vivo bioluminescent imaging of influenza a virus infection and characterization of novel cross-protective monoclonal antibodies.Journal of virology · 2013Article
- CD200 receptor controls sex-specific TLR7 responses to viral infection.PLoS pathogens · 2012Article
- The proteasome inhibitor Velcade enhances rather than reduces disease in mouse hepatitis coronavirus-infected mice.Journal of virology · 2010Article
- Type I interferon receptor-independent and -dependent host transcriptional responses to mouse hepatitis coronavirus infection in vivo.BMC genomics · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bioluminescence imaging (BLI) is a powerful new method to study virus dissemination in the live animal. Here we used this method to monitor the spatial and temporal progression of mouse hepatitis coronavirus (MHV) infection in mice using luciferase-expressing viruses. Upon intranasal inoculation, virus replication could initially be observed in the nasal cavity and the cervical lymph nodes, after which the infection spread to the brain and frequently to the eyes. The kinetics of virus spread to and clearance from the brain appeared to depend on the inoculation dose. After intraperitoneal inoculation, virus replication was predominantly observed in the liver and occasionally in the intestines, but interestingly also in the tail and paws. BLI thus elucidated new anatomic locations of virus replication. Furthermore, MHV dissemination was shown to be critically depended on the viral spike protein, but also on the mouse strain used. Widespread dissemination was observed in mice lacking a functional type I interferon response. The importance of the type I interferon system in limiting viral spread was also demonstrated by the administration of type I interferons to mice. Our results provide new insights in coronavirus pathogenesis and demonstrate the potential of BLI to study coronavirus-host interactions in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.