ArticleThe EMBO journal2009
Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.
Article in The EMBO journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 75 citations in OpenAlex.
- Fission yeast RPA-TERT-Tpz1TPP1 complex promotes telomere extension and suppresses telomere recombination.PLoS genetics · 2026Article
- Fission yeast RPA-TERT-Tpz1bioRxiv : the preprint server for biology · 2026Article
- Pot1 promotes telomere DNA replication via the Stn1-Ten1 complex in fission yeast.Nucleic acids research · 2023Article
- A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells.Nucleic acids research · 2022Article
- GGGCTA repeats can fold into hairpins poorly unfolded by replication protein A: a possible origin of the length-dependent instability of GGGCTA variant repeats in human telomeres.Nucleic acids research · 2021Article
- Ssu72 phosphatase is a conserved telomere replication terminator.The EMBO journal · 2019Article
- Review
- Tpz1Communications biology · 2019Article
- Precarious maintenance of simple DNA repeats in eukaryotes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Telomerase Mechanism of Telomere Synthesis.Annual review of biochemistry · 2017Review
- Solving the Telomere Replication Problem.Genes · 2017Review
- TeloPCR-seq: a high-throughput sequencing approach for telomeres.FEBS letters · 2016Article
- Timeless protection of telomeres.Current genetics · 2016Review
- Swi1Timeless Prevents Repeat Instability at Fission Yeast Telomeres.PLoS genetics · 2016Article
- Ccq1-Tpz1TPP1 interaction facilitates telomerase and SHREC association with telomeres in fission yeast.Molecular biology of the cell · 2015Article
- RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends.The EMBO journal · 2015Article
- Chromatin immunoprecipitation to detect DNA replication and repair factors.Methods in molecular biology (Clifton, N.J.) · 2015Article
- POT1a and components of CST engage telomerase and regulate its activity in Arabidopsis.PLoS genetics · 2014Article
- Tpz1-Ccq1 and Tpz1-Poz1 interactions within fission yeast shelterin modulate Ccq1 Thr93 phosphorylation and telomerase recruitment.PLoS genetics · 2014Article
- Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association.EMBO reports · 2014Article
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6 authors at 2 institutions in 1 country.
Funding
Abstract
To maintain genomic integrity, telomeres must undergo switches from a protected state to an accessible state that allows telomerase recruitment. To better understand how telomere accessibility is regulated in fission yeast, we analysed cell cycle-dependent recruitment of telomere-specific proteins (telomerase Trt1, Taz1, Rap1, Pot1 and Stn1), DNA replication proteins (DNA polymerases, MCM, RPA), checkpoint protein Rad26 and DNA repair protein Nbs1 to telomeres. Quantitative chromatin immunoprecipitation studies revealed that MCM, Nbs1 and Stn1 could be recruited to telomeres in the absence of telomere replication in S-phase. In contrast, Trt1, Pot1, RPA and Rad26 failed to efficiently associate with telomeres unless telomeres are actively replicated. Unexpectedly, the leading strand DNA polymerase epsilon (Polepsilon) arrived at telomeres earlier than the lagging strand DNA polymerases alpha (Polalpha) and delta (Poldelta). Recruitment of RPA and Rad26 to telomeres matched arrival of DNA Polepsilon, whereas S-phase specific recruitment of Trt1, Pot1 and Stn1 matched arrival of DNA Polalpha. Thus, the conversion of telomere states involves an unanticipated intermediate step where lagging strand synthesis is delayed until telomerase is recruited.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.