ArticleAmerican journal of physiology. Cell physiology2009
Membrane targeting and intracellular trafficking of the human sodium-dependent multivitamin transporter in polarized epithelial cells.
Article in American journal of physiology. Cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- Biotin-mediated drug delivery: does the biotin transporter mediate uptake of biotin conjugates?Journal of enzyme inhibition and medicinal chemistry · 2025Article
- Transporters in vitamin uptake and cellular metabolism: impacts on health and disease.Life metabolism · 2025Review
- Review
- The Effect of Maternal Probiotic or Synbiotic Supplementation on Sow and Offspring Gastrointestinal Microbiota, Health, and Performance.Animals : an open access journal from MDPI · 2023Review
- Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like Phenotype.Nutrients · 2023Article
- PLEKHA5, PLEKHA6, and PLEKHA7 bind to PDZD11 to target the Menkes ATPase ATP7A to the cell periphery and regulate copper homeostasis.Molecular biology of the cell · 2021Article
- Effect of chronic alcohol exposure on gut vitamin B7 uptake: involvement of epigenetic mechanisms and effect of alcohol metabolites.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Upregulation of Vitamin C Transporter Functional Expression in 5xFAD Mouse Intestine.Nutrients · 2021Article
- Tamoxifen-induced, intestinal-specific deletion ofAmerican journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Lipopolysaccharide inhibits colonic biotin uptake via interference with membrane expression of its transporter: a role for a casein kinase 2-mediated pathway.American journal of physiology. Cell physiology · 2017Article
- Mutations in SLC5A6 associated with brain, immune, bone, and intestinal dysfunction in a young child.Human genetics · 2017Article
- Role of the sodium-dependent multivitamin transporter (SMVT) in the maintenance of intestinal mucosal integrity.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Interaction of α-Lipoic Acid with the Human Na+/Multivitamin Transporter (hSMVT).The Journal of biological chemistry · 2015Article
- The sodium/multivitamin transporter: a multipotent system with therapeutic implications.Vitamins and hormones · 2015Review
- Molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter.The Journal of biological chemistry · 2014Article
- Recent advances in transport of water-soluble vitamins in organs of the digestive system: a focus on the colon and the pancreas.American journal of physiology. Gastrointestinal and liver physiology · 2013Review
- Caco-2 cell acquisition of dietary iron(III) invokes a nanoparticulate endocytic pathway.PloS one · 2013Article
- Mitochondrial uptake of thiamin pyrophosphate: physiological and cell biological aspects.PloS one · 2013Article
- Human Organic Solute Transporter (hOST): protein interaction and membrane sorting process.International journal of biochemistry and molecular biology · 2012Article
- Cys(294) is essential for the function of the human sodium-dependent multivitamin transporter.Biochimica et biophysica acta · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The human sodium-dependent multivitamin transporter (hSMVT) mediates sodium-dependent uptake of biotin in renal and intestinal epithelia. To date, however, there is nothing known about the structure-function relationship or targeting sequences in the hSMVT polypeptide that control its polarized expression within epithelia. Here, we focused on the role of the COOH-terminal tail of hSMVT in the targeting and functionality of this transporter. A full-length hSMVT-green fluorescent protein (GFP) fusion protein was functional and expressed at the apical membrane in renal and intestinal cell lines. Microtubule disrupting agents disrupted the mobility of trafficking vesicles and impaired cell surface delivery of hSMVT, which was also prevented in cells treated with dynamitin (p50), brefeldin, or monensin. Progressive truncation of the COOH-terminal tail impaired the functionality and targeting of the transporter. First, biotin transport decreased by approximately 20-30% on deletion of up to 15 COOH-terminal amino acids of hSMVT, a decrease mimicked solely by deletion of the terminal PDZ motif (TSL). Second, deletions into the COOH-terminal tail (between residues 584-612, containing a region of predicted high surface accessibility) resulted in a further drop in hSMVT transport (to approximately 40% of wild-type). Third, apical targeting was lost on deletion of a helical-prone region between amino acids 570-584. We conclude that the COOH tail of hSMVT contains several determinants important for polarized targeting and biotin transport.
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