Evidence map›Paper›PMID 19211770›Full record

ArticleJournal of virology2009

Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses.

Yuning Sun, Aaron Yun Chen, Fang Cheng, Wuxiang Guan, F Brent Johnson, Jianming Qiu

Open access · greenAbstract read
In one paragraph

Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.

0numbers the graph read from it
0cells of the map it votes in
98citing papers in PubMed
8.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

98 citing papers in PubMed, 153 citations in OpenAlex.

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  17. Old and Novel Enteric Parvoviruses of Dogs.Pathogens (Basel, Switzerland) · 2023
    Review
  18. Article
  19. Article
  20. Review

38 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yuning SunDepartment of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Blvd., Kansas City, KS 66160, USA.
Aaron Yun Chen
Fang Cheng
Wuxiang Guan
F Brent Johnson
Jianming Qiu
University of Kansas Medical Center · USBrigham Young University · US

Funding

XRAY CRYSTALLOGRAPHY CORE (JANUARY 1 - JUNE 30, 2005)P20RR016443 · NCRR · UNIVERSITY OF KANSAS MEDICAL CENTER · PI VINES, CHARLOTTE M · 2001 to 2010
$21.3M
Post-Transcriptional Regulation of Parvovirus Capsid Gene ExpressionR01AI070723 · NIAID · UNIVERSITY OF KANSAS MEDICAL CENTER · PI QIU, JIANMING · 2007 to 2017
$3.3M
NCRR NIH HHS P20 RR016443NIAID NIH HHS R01 AI070723
6 · The paper itself

Abstract

Minute virus of canines (MVC) is a member of the genus Bocavirus in the family Parvoviridae. We have molecularly cloned and sequenced the 5'- and 3'-terminal palindromes of MVC. The MVC genome, 5,404 nucleotides (nt) in length, shared an identity of 52.6% and 52.1% with that of human bocavirus and bovine parvovirus, respectively. It had distinct palindromic hairpins of 183 nt and 198 nt at the left-end and right-end termini of the genome, respectively. The left-end terminus was also found in two alternative orientations (flip or flop). Both termini shared extensive similarities with those of bovine parvovirus. Four full-length molecular clones constructed with different orientations of the left-end terminus proved to be infectious in Walter Reed canine cell/3873D (WRD) canine cells. Both MVC infection and transfection of the infectious clone in WRD cells revealed an identical RNA transcription profile that was similar to that of bovine parvovirus. Mutagenesis of the infectious clone demonstrated that the middle open reading frame encodes the NP1 protein. This protein, unique to the genus Bocavirus, was essential for MVC DNA replication. Moreover, the phospholipase A2 motif in the VP1 unique region was also critical for MVC infection. Thus, our studies revealed important information about the genus Bocavirus that may eventually help us to clone the human bocavirus and study its pathogenesis.

Indexed as

Virus ReplicationAnimalsBocavirusCell LineCloning, MolecularDNA Mutational AnalysisDNA, ViralDogsInverted Repeat SequencesMolecular Sequence DataOpen Reading FramesRNA PrecursorsSequence Analysis, DNASequence Homology, Nucleic AcidTranscription, GeneticViral ProteinsDNA, ViralRNA PrecursorsViral Proteins

Identifiers

PMID19211770
PMCPMC2663281
OpenAlexW2145051154

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.