ArticleThe Journal of biological chemistry2009
The mechanism of photo-energy storage in the Halorhodopsin chloride pump.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- A Unique Light-Driven Proton Transportation Signal in Halorhodopsin from Natronomonas pharaonis.Biophysical journal · 2016Article
- Beta Cell Hubs Dictate Pancreatic Islet Responses to Glucose.Cell metabolism · 2016Article
- Energy utilization in fluctuating biological energy converters.Structural dynamics (Melville, N.Y.) · 2016Article
- Electrostatic interactions and hydrogen bond dynamics in chloride pumping by halorhodopsin.Biochimica et biophysica acta · 2014Article
- Catalytic strategy used by the myosin motor to hydrolyze ATP.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The photochemical determinants of color vision: revealing how opsins tune their chromophore's absorption wavelength.BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Review
- Optical control of neuronal activity.Annual review of biophysics · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The light-driven pump Halorhodopsin (hR) uses the energy stored in an initial meta-stable state (K), in which the bound retinal chromophore has been photoisomerized from all-trans to 13-cis, to drive the translocation of one chloride anion across the membrane. Thus far it was unclear whether retinal twisting or charge separation between the positive Schiff base of the retinal and the chloride anion is the primary mechanism of energy storage. Here, combined quantum mechanical/molecular mechanical (QM/MM) simulations show that the energy is predominantly stored by charge separation. However, a large variability in retinal twisting is observed, thus reconciling the contradictory hypotheses for storage. Surprisingly, the energy stored in the K-state amounts to only one-fifth of the photon energy. We explain why the protein cannot store more: even though this would accelerate chloride pumping, raising the K-state also reduces the relative energy barriers against unproductive decay, in particular via the premature cis to trans back-isomerization. Indeed, the protein has maximized its storage so that the back-isomerization barriers are just high enough (> or =18 kcal/mol) to keep the decay rate (1/100 ms) slower than the remaining photocycle (1/20 ms). This need to stabilize the captured photon-energy until it can be used in subsequent steps is inherent to light-driven proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.