ArticleCardiovascular pathology : the official journal of the Society for Cardiovascular Pathology
Creation, validation, and quantitative analysis of protein expression in vascular tissue microarrays.
Article in Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- The Movember Global Action Plan 1 (GAP1): Unique Prostate Cancer Tissue Microarray Resource.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2022Article
- Tissue Transglutaminase Modulates Vascular Stiffness and Function Through Crosslinking-Dependent and Crosslinking-Independent Functions.Journal of the American Heart Association · 2017Article
- Complex Sources of Variation in Tissue Expression Data: Analysis of the GTEx Lung Transcriptome.American journal of human genetics · 2016Article
- Postmortem cardiac tissue maintains gene expression profile even after late harvesting.BMC genomics · 2012Article
- Quantitative analysis of p53 expression in human normal and cancer tissue microarray with global normalization method.International journal of clinical and experimental pathology · 2011Article
- Breast cancer diagnosis using a microfluidic multiplexed immunohistochemistry platform.PloS one · 2010Article
- A comprehensive histopathological evaluation of vascular medial fibrosis: insights into the pathophysiology of arterial stiffening.Atherosclerosis · 2010Article
- Immunohistochemistry in diagnostic surgical pathology: contributions of protein life-cycle, use of evidence-based methods and data normalization on interpretation of immunohistochemical stains.International journal of clinical and experimental pathology · 2009Review
- Glomerular protein levels of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 are lower in diabetic subjects.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Use of human vascular tissue microarrays for measurement of advanced glycation endproducts.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Tissue inhibitor of matrix metalloproteinase-3 levels in the extracellular matrix of lung, kidney, and eye increase with age.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Dermal type I collagen assessment by digital image analysis.Anais brasileiros de dermatologiaArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundTissue microarrays (TMAs) are collections of multiple tissue cores placed in parallel in a single acceptor block and traditionally used to investigate protein expression in neoplastic tissues. We validated the use of TMAs to investigate protein expression in vascular segments.
methodsVascular tissues were collected from 100 adult subjects undergoing autopsy. A diverse set of vessels were harvested and arrayed over 17 TMAs. A total of 1377 unique tissues, each with a 1.5-mm feature size, were analyzed using histochemical and immunohistochemical (IHC) diaminobenzidine (DAB) methods.
resultsHistomorphometric analysis of vascular disease demonstrated the TMA features captured the majority of the vascular alterations (intimal hyperplasia and atherosclerosis) seen in the original blood vessel section. Measurements of IHC staining intensity based on color deconvolution were used to quantify antigen abundance in defined regions of interest (ROI). Validation was performed using antibodies to connective tissue growth factor (CTGF), receptor for advanced glycation end products (AGER/RAGE), and matrix metalloproteinase 3 (MMP-3). IHC staining was highly correlated between duplicate features from the same vascular site over these three proteins.
conclusionThis study validates the use of TMA technology to investigate the vascular wall utilizing staining intensity data.
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