ArticleMolecular microbiology2009
Differential expression of a virulence factor in pathogenic and non-pathogenic mycobacteria.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Protein kinase G-a key regulator of pathogenesis in Mycobacterium tuberculosis infection.Archives of microbiology · 2025Review
- Deletion ofAntimicrobial agents and chemotherapy · 2021Article
- Macrophage-microbe interaction: lessons learned from the pathogen Mycobacterium tuberculosis.Seminars in immunopathology · 2018Review
- Host-pathogen redox dynamics modulate Mycobacterium tuberculosis pathogenesis.Pathogens and disease · 2018Article
- PknG supports mycobacterial adaptation in acidic environment.Molecular and cellular biochemistry · 2018Article
- In vivo and in vitro expression of five genes involved in Corynebacterium pseudotuberculosis virulence.AMB Express · 2018Article
- PknG senses amino acid availability to control metabolism and virulence of Mycobacterium tuberculosis.PLoS pathogens · 2017Article
- Mycobacterium bovis BCG Interferes with miR-3619-5p Control of Cathepsin S in the Process of Autophagy.Frontiers in cellular and infection microbiology · 2016Article
- Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3.Nature communications · 2015Article
- RD-1 encoded EspJ protein gets phosphorylated prior to affect the growth and intracellular survival of mycobacteria.Scientific reports · 2015Article
- A redox regulatory system critical for mycobacterial survival in macrophages and biofilm development.PLoS pathogens · 2015Article
- Article
- Striking the Right Balance Determines TB or Not TB.Frontiers in immunology · 2014Review
- Functional characterization delineates that a Mycobacterium tuberculosis specific protein kinase (Rv3080c) is responsible for the growth, phagocytosis and intracellular survival of avirulent mycobacteria.Molecular and cellular biochemistry · 2012Article
- Association between vitamin D insufficiency and tuberculosis in a Vietnamese population.BMC infectious diseases · 2010Article
- Temperature-dependent regulation of mycolic acid cyclopropanation in saprophytic mycobacteria: role of the Mycobacterium smegmatis 1351 gene (MSMEG_1351) in CIS-cyclopropanation of alpha-mycolates.The Journal of biological chemistry · 2010Article
- Carbon metabolism of intracellular bacterial pathogens and possible links to virulence.Nature reviews. Microbiology · 2010Review
- Downregulation of protein kinase C-alpha enhances intracellular survival of Mycobacteria: role of PknG.BMC microbiology · 2009Article
- Key residues in Mycobacterium tuberculosis protein kinase G play a role in regulating kinase activity and survival in the host.The Journal of biological chemistry · 2009Article
- Protein kinase G is required for intrinsic antibiotic resistance in mycobacteria.Antimicrobial agents and chemotherapy · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pathogenicity of mycobacterial infections depends on virulence factors that mediate survival inside host macrophages. These virulence factors are generally believed to be specific for pathogenic species and absent or mutated in non-pathogenic strains. The serine/threonine protein kinase G (PknG) mediates survival of mycobacteria within macrophages by blocking lysosomal delivery. Here we describe a gene of the non-pathogenic species Mycobacterium smegmatis that is 78% identical with pknG of Mycobacterium tuberculosis and M. bovis bacillus Calmette-Guérin (BCG). When cloned into expression vectors, the M. smegmatis pknG orthologue produced an active kinase and performed the same function as its M. bovis BCG counterpart in intracellular survival. In addition, similar levels of pknG transcripts were found in M. bovis BCG and M. smegmatis. However, virtually no translation product of chromosomal pknG could be detected in M. smegmatis both after in vitro growth and after macrophage infection. This lack of efficient translation was shown to be caused by regulatory elements in the upstream region of the M. smegmatis gene. The data reveal dramatically increased translational efficiency of a virulence gene in a pathogenic mycobacterium compared with a non-pathogenic mycobacterium suggesting that changes in expression levels may underlie evolution of pknG and other pathogenicity genes in mycobacterium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.