ArticleLab on a chip2009
Textural guidance cues for controlling process outgrowth of mammalian neurons.
Article in Lab on a chip, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
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- Electron Microscopy of Neurons on Biomimetic Substrates.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Dynamics of Endothelial Engagement and Filopodia Formation in Complex 3D Microscaffolds.International journal of molecular sciences · 2022Article
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- Anisotropic scaffolds for peripheral nerve and spinal cord regeneration.Bioactive materials · 2021Review
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- Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions.Nanoscale advances · 2020Article
- Proliferation and Cluster Analysis of Neurons and Glial Cell Organization on Nanocolumnar TiN Sub-Strates.International journal of molecular sciences · 2020Article
- Enzymatically crosslinked gelatin-laminin hydrogels for applications in neuromuscular tissue engineering.Biomaterials science · 2020Article
- Cellular and Subcellular Contact Guidance on Microfabricated Substrates.Frontiers in bioengineering and biotechnology · 2020Review
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- High-Aspect-Ratio Semiconducting Polymer Pillars for 3D Cell Cultures.ACS applied materials & interfaces · 2019Article
- Engineering small tubes with changes in diameter for the study of kidney cell organization.Biomicrofluidics · 2018Article
- Characterization of dorsal root ganglion neurons cultured on silicon micro-pillar substrates.Scientific reports · 2016Article
- Large-scale topographical screen for investigation of physical neural-guidance cues.Scientific reports · 2015Article
- The effect of substrate topography on direct reprogramming of fibroblasts to induced neurons.Biomaterials · 2014Article
- Substrates coated with silver nanoparticles as a neuronal regenerative material.International journal of nanomedicine · 2014Article
- Superimposed topographic and chemical cues synergistically guide neurite outgrowth.Lab on a chip · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We explore textural cues as a mechanism for controlling neuronal process outgrowth in primary cultures of mammalian neurons. The work uses a form of decal transfer lithography to generate arrays of PDMS posts of various dimensions and spacings on glass substrates that are rendered growth-compliant by subsequent treatment with a protein activator. Hippocampal neurons plated on these substrates are used to determine how the posts direct process growth by acting as attachment points or guidance cues. Textural features varying over a large range, even as large as 100 microm in diameter, dramatically affect process growth. Indeed, two growth regimes are observed; at the smaller feature sizes considered, process branching strongly aligns (at right angles) along the post mesh, while neuronal outgrowth on the larger feature sizes elicits process wrapping. The latter behavior most strongly manifests in neurons plated initially at approximately 100 cells/mm(2), where the cells were able to form networks, while for isolated neurons, the cells exhibit poorer viability and development. Bag cell neurons from Aplysia californica also display regular growth patterns, but in this case are guided by contact avoidance of the posts, a behavior qualitatively different than that of the hippocampal neurons.
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Registered trials
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