ArticleNucleic acids research2009
Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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Who cites it
108 citing papers in PubMed, 253 citations in OpenAlex.
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- Natural diversity-guided catalytic-core chimerism engineers a rapid and inhibitor-tolerant reverse transcriptase.Journal of biological engineering · 2026Article
- Research progress on direct PCR and its applications.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Review
- Assessment of the effectiveness of host depletion techniques for profiling fish skin microbiomes and metagenomic analysis.Microbiology spectrum · 2026Article
- Mechanism and application of Taq DNA polymerase in TaqMan qPCR.Frontiers in bioengineering and biotechnology · 2026Article
- Innovative Rapid Detection System for Nondeletion-Type α‑Thalassemia via Whole-Blood Amplification and Microfluidic Technology.ACS omega · 2025Article
- Comparison of boiling versus magnetic bead techniques in nucleic acid extraction for human papillomavirus detection: evidence based 17,179 cases.Virology journal · 2025Article
- Towards practical point-of-care quick, ubiquitous, integrated, cost-efficient molecular diagnostic Kit (QUICK) PCR for future pandemic response.Microsystems & nanoengineering · 2025Review
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- Simple dual filter workflow for facilitating blood culture-free and sensitive detection of pathogenic bacteria from blood.Scientific reports · 2025Article
- Lyophilised Reverse Transcriptase and Polymerase for Point-of-Care Diagnostics of SARS-CoV-2.Microbial biotechnology · 2025Article
- Cas12a Cis-cleavage mediated lateral flow assay enables multiplex and ultra-specific nucleic acid detection.Nature communications · 2025Article
- Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors.Frontiers in bioengineering and biotechnology · 2025Article
- Long Amplicon Nanopore Sequencing for Dual-Typing RdRp and VP1 Genes of Norovirus Genogroups I and II in Wastewater.Food and environmental virology · 2024Article
- Enhanced Recovery and Detection of Highly Infectious Animal Disease Viruses by Virus Capture Using NanotrapViruses · 2024Article
- Production of Reverse Transcriptase and DNA Polymerase in Bacterial Expression Systems.Bioengineering (Basel, Switzerland) · 2024Article
- PCR in Forensic Science: A Critical Review.Genes · 2024Review
- A Fusion of Taq DNA Polymerase with the CL7 Protein fromMolecules (Basel, Switzerland) · 2024Article
48 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Potent PCR inhibitors in blood and soil samples can cause false negative results from PCR-based clinical and forensic tests. We show that the effect of these inhibitors is primarily upon Taq DNA polymerase, since mutational alteration of the polymerase can overcome the inhibition to the extent that no DNA purification is now required. An N-terminal deletion (Klentaq1) is some 10-100-fold inhibition resistant to whole blood compared to full-length, wild-type (w.t.) Taq, which is strongly inhibited by 0.1-1% blood. Further mutations at codon 708, both in Klentaq 1 and Taq, confer enhanced resistance to various inhibitors of PCR reactions, including whole blood, plasma, hemoglobin, lactoferrin, serum IgG, soil extracts and humic acid, as well as high concentrations of intercalating dyes. Blood PCR inhibitors can predominantly reduce the DNA extension speed of the w.t. Taq polymerase as compared to the mutant enzymes. Single-copy human genomic targets are readily amplified from whole blood or crude soil extract, without pretreatment to purify the template DNA, and the allowed increase in dye concentration overcomes fluorescence background and quenching in real-time PCR of blood.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.