ArticleThe Journal of biological chemistry2009
Stimulation of UvrD helicase by UvrAB.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- The Role of SF1 and SF2 Helicases in Biotechnological Applications.Applied biochemistry and biotechnology · 2024Review
- DNA-Unwinding Dynamics of Escherichia coli UvrD Lacking the C-Terminal 40 Amino Acids.Biophysical journal · 2020Article
- Direct removal of RNA polymerase barriers to replication by accessory replicative helicases.Nucleic acids research · 2019Article
- Regulation of UvrD Helicase Activity by MutL.Journal of molecular biology · 2018Article
- The structure and function of an RNA polymerase interaction domain in the PcrA/UvrD helicase.Nucleic acids research · 2017Article
- Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli.Journal of bacteriology · 2016Article
- Active displacement of RecA filaments by UvrD translocase activity.Nucleic acids research · 2015Article
- Investigation of bacterial nucleotide excision repair using single-molecule techniques.DNA repair · 2014Review
- Protein-DNA complexes are the primary sources of replication fork pausing in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Prokaryotic nucleotide excision repair.Cold Spring Harbor perspectives in biology · 2013Review
- The conserved C-terminus of the PcrA/UvrD helicase interacts directly with RNA polymerase.PloS one · 2013Article
- RECQL5 plays co-operative and complementary roles with WRN syndrome helicase.Nucleic acids research · 2013Article
- Structural and mechanistic insight into DNA unwinding by Deinococcus radiodurans UvrD.PloS one · 2013Article
- The AddAB helicase-nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain.Nucleic acids research · 2011Article
- Interaction of Rep and DnaB on DNA.Nucleic acids research · 2011Article
- Localization of an accessory helicase at the replisome is critical in sustaining efficient genome duplication.Nucleic acids research · 2011Article
- DNA Helicases.EcoSal Plus · 2010Article
- Modulation of UvrD helicase activity by covalent DNA-protein cross-links.The Journal of biological chemistry · 2010Article
- A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase.The Journal of biological chemistry · 2010Article
- The unstructured C-terminal extension of UvrD interacts with UvrB, but is dispensable for nucleotide excision repair.DNA repair · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Helicases play critical roles in all aspects of nucleic acid metabolism by catalyzing the remodeling of DNA and RNA structures. UvrD is an abundant helicase in Escherichia coli with well characterized functions in mismatch and nucleotide excision repair and a possible role in displacement of proteins such as RecA from single-stranded DNA. The mismatch repair protein MutL is known to stimulate UvrD. Here we show that the nucleotide excision repair proteins UvrA and UvrB can together stimulate UvrD-catalyzed unwinding of a range of DNA substrates containing strand discontinuities, including forked DNA substrates. The stimulation is specific for UvrD, as UvrAB failed to stimulate Rep helicase, a UvrD homologue. Moreover, although UvrAB can promote limited strand displacement, stimulation of UvrD did not require the strand displacement function of UvrAB. We conclude that UvrAB, like MutL, modulate UvrD helicase activity. This stimulation likely plays a role in DNA strand and protein displacement by UvrD in nucleotide excision repair. Promotion of UvrD-catalyzed unwinding of nicked duplexes by UvrAB may also explain the need for UvrAB and UvrD in Okazaki fragment processing in cells lacking DNA polymerase I. More generally, these data support the idea that helicase activity is regulated in vivo, with helicases acting as part of multisubunit complexes rather than in isolation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.