ArticleMicroscopy research and technique2009
Fluorescence recovery after photobleaching and photoconversion in multiple arbitrary regions of interest using a programmable array microscope.
Article in Microscopy research and technique, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 32 citations in OpenAlex.
- Correlative scanning electron and super-resolution structured illumination microscopy.bioRxiv : the preprint server for biology · 2026Article
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- Artifact-free whole-slide imaging with structured illumination microscopy and Bayesian image reconstruction.GigaScience · 2020Article
- Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction.GigaScience · 2019Article
- Quantitative super-resolution single molecule microscopy dataset of YFP-tagged growth factor receptors.GigaScience · 2018Article
- erbB3 is an active tyrosine kinase capable of homo- and heterointeractions.Molecular and cellular biology · 2014Article
- Tonic signaling from O₂ sensors sets neural circuit activity and behavioral state.Nature neuroscience · 2012Article
- Caught in the act: quantifying protein behaviour in living cells.Trends in cell biology · 2009Review
- Watching the assembly of an organ a single cell at a time using confocal multi-position photoactivation and multi-time acquisition.Organogenesis · 2009Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Photomanipulation (photobleaching, photoactivation, or photoconversion) is an essential tool in fluorescence microscopy. Fluorescence recovery after photobleaching (FRAP) is commonly used for the determination of lateral diffusion constants of membrane proteins, and can be conveniently implemented in confocal laser scanning microscopy (CLSM). Such determinations provide important information on molecular dynamics in live cells. However, the CLSM platform is inherently limited for FRAP because of its inflexible raster (spot) scanning format. We have implemented FRAP and photoactivation protocols using structured illumination and detection in a programmable array microscope (PAM). The patterns are arbitrary in number and shape, dynamic and adjustable to and by the sample characteristics. We have used multispot PAM-FRAP to measure the lateral diffusion of the erbB3 (HER3) receptor tyrosine kinase labeled by fusion with mCitrine on untreated cells and after treatment with reagents that perturb the cytoskeleton or plasma membrane or activate coexpressed erbB1 (HER1, the EGF receptor EGFR). We also show the versatility of the PAM for photoactivation in arbitrary regions of interest, in cells expressing erbB3 fused with the photoconvertible fluorescent protein dronpa.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.