ArticleCellular microbiology2009
CfaE tip mutations in enterotoxigenic Escherichia coli CFA/I fimbriae define critical human intestinal binding sites.
Article in Cellular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 48 citations in OpenAlex.
- Preparation of Bispecific IgY-scFvs Inhibition Adherences of EnterotoxigenicInternational journal of molecular sciences · 2024Article
- Efficacy Evaluation of an Intradermally Delivered EnterotoxigenicMicroorganisms · 2024Article
- The role of the minor colonization factor CS14 in adherence to intestinal cell models by geographically diverse ETEC isolates.mSphere · 2023Article
- The role of CFA/I in adherence and toxin delivery by ETEC expressing multiple colonization factors in the human enteroid model.PLoS neglected tropical diseases · 2022Article
- Comparative Genomics Applied to Systematically Assess Pathogenicity Potential in Shiga Toxin-ProducingMicroorganisms · 2022Article
- Development and Comparison of a Panel of Modified CS17 Fimbrial Tip Adhesin Proteins as Components for an Adhesin-Based Vaccine against EnterotoxigenicMicroorganisms · 2021Article
- Anti-CfaE nanobodies provide broad cross-protection against major pathogenic enterotoxigenic Escherichia coli strains, with implications for vaccine design.Scientific reports · 2021Article
- Phosphodiesterase 5 (PDE5) restricts intracellular cGMP accumulation during enterotoxigenicGut microbes · 2020Article
- Cross-Reactivity, Epitope Mapping, and Potency of Monoclonal Antibodies to Class 5 Fimbrial Tip Adhesins of Enterotoxigenic Escherichia coli.Infection and immunity · 2020Article
- Biochemical and immunological characterization of an ETEC CFA/I adhesin cholera toxin B subunit chimera.PloS one · 2020Article
- Intradermal or Sublingual Delivery and Heat-Labile Enterotoxin Proteins Shape Immunologic Responses to a CFA/I Fimbria-Derived Subunit Antigen Vaccine against Enterotoxigenic Escherichia coli.Infection and immunity · 2019Article
- A tale of two bacterial enteropathogens and one multivalent vaccine.Cellular microbiology · 2019Review
- Biochemical and Immunological Evaluation of Recombinant CS6-Derived Subunit Enterotoxigenic Escherichia coli Vaccine Candidates.Infection and immunity · 2019Article
- A roadmap for enterotoxigenicHuman vaccines & immunotherapeutics · 2019Review
- Identification and Characterization of Human Monoclonal Antibodies for Immunoprophylaxis against Enterotoxigenic Escherichia coli Infection.Infection and immunity · 2018Article
- Glyco-engineered cell line and computational docking studies reveals enterotoxigenic Escherichia coli CFA/I fimbriae bind to Lewis a glycans.Scientific reports · 2018Article
- Highly conserved type 1 pili promote enterotoxigenic E. coli pathogen-host interactions.PLoS neglected tropical diseases · 2017Article
- Article
- Binding of CFA/I Pili of Enterotoxigenic Escherichia coli to Asialo-GM1 Is Mediated by the Minor Pilin CfaE.Infection and immunity · 2016Article
- Simple method for purification of enterotoxigenic Escherichia coli fimbriae.Protein expression and purification · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Enterotoxigenic Escherichia coli (ETEC) use colonization factors to attach to the human intestinal mucosa, followed by enterotoxin expression that induces net secretion and diarrhoeal illness. ETEC strain H10407 expresses CFA/I fimbriae, which are composed of multiple CfaB structural subunits and a CfaE tip subunit. Currently, the contribution of these individual fimbrial subunits in intestinal binding remains incompletely defined. To identify the role of CfaE in attachment in the native ETEC background, an R181A single-amino-acid substitution was introduced by recombination into the H10407 genome. The substitution of R181A eliminated haemagglutination and binding of intestinal mucosa biopsies in in vitro organ culture assays, without loss of CFA/I fimbriae expression. Wild-type in trans plasmid-expressed cfaE restored the binding phenotype. In contrast, in trans expression of cfaE containing amino acid 181 substitutions with similar amino acids, lysine, methionine and glutamine did not restore the binding phenotype, indicating that the loss of the binding phenotype was due to localized areas of epitope disruption. R181 appears to have an irreplaceable role in the formation of a receptor-binding feature on CFA/I fimbriae. The results specifically indicate that the CfaE tip protein is a required binding factor in CFA/I-mediated ETEC colonization, making it a potentially important vaccine antigen.
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