ArticleTissue engineering. Part A2009
In vitro 3D model for human vascularized adipose tissue.
Article in Tissue engineering. Part A, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed, 2 syntheses or guidelines pooled it, 150 citations in OpenAlex.
- Preclinical techniques to investigate exercise training in vascular pathophysiology.American journal of physiology. Heart and circulatory physiology · 2021Pooled it
- Potential drawbacks in cell-assisted lipotransfer: A systematic review of existing reports (Review).Molecular medicine reports · 2016Pooled it
- Comparative analysis of select 3D human mesenchymal stromal cell-derived spheroid models for adipogenic differentiation.Adipocyte · 2026Article
- 3D Bioprinting for Engineered Tissue Constructs and Patient-Specific Models: Current Progress and Prospects in Clinical Applications.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Mouse vascularized adipose spheroids: an organotypic model for thermogenic adipocytes.Frontiers in endocrinology · 2024Article
- Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications.Frontiers in endocrinology · 2024Review
- Development of a More Environmentally Friendly Silk Fibroin Scaffold for Soft Tissue Applications.Journal of functional biomaterials · 2023Article
- Spheroids derived from the stromal vascular fraction of adipose tissue self-organize in complex adipose organoids and secrete leptin.Stem cell research & therapy · 2023Article
- Article
- Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies.Biomolecules · 2022Article
- Modelling metabolic diseases and drug response using stem cells and organoids.Nature reviews. Endocrinology · 2022Review
- High-throughput drug screening models of mature adipose tissues which replicate the physiology of patients' Body Mass Index (BMI).Bioactive materials · 2022Article
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix.Biomimetics (Basel, Switzerland) · 2021Article
- Review
- Development of novel human in vitro vascularized adipose tissue model with functional macrophages.Cytotechnology · 2020Article
- Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model.Bioengineering (Basel, Switzerland) · 2020Article
- Tissue engineering and regenerative medicine strategies for the female breast.Journal of tissue engineering and regenerative medicine · 2020Review
- Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease.Frontiers in bioengineering and biotechnology · 2020Review
- Human Adipose Derived Cells in Two- and Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct.Stem cells international · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The clinical need for both three-dimensional (3D) soft tissue replacements and in vitro adipose tissue models continues to grow. In this study, we evaluated structural and functional characteristics of an in vitro 3D coculture model of vascularized adipose tissue. Tomato red-infected human adipose tissue-derived mesenchymal stem cells (hASCs) and green fluorescence protein-infected human umbilical vein endothelial cells were cocultured on 3D aqueous-derived silk scaffolds for 2 weeks. Confocal microscopy images demonstrated viability of cocultures and organization of both cell types over time. Endothelial cells aligned with time, and further histological analyses revealed continuous endothelial lumen formation in both differentiated and undifferentiated cocultures. Differentiated adipose cocultures secreted significantly higher levels of leptin than undifferentiated cocultures at 1 and 2 weeks. Additionally, lipid accumulation was demonstrated with Oil Red O staining, where positive staining was higher in the differentiated cocultures. A promising in vitro approach for the vascularization of tissue-engineered adipose tissue, and the ability to vascularize a construct containing hASCs was demonstrated. The strategy outlined provides a basis for the formation of other in vitro vascularized tissues as well as a path forward for the sustainable formation of soft tissue due to the use of slowly degrading silk scaffolds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.