ArticleNMR in biomedicine2009
A robust protocol for regional evaluation of methacholine challenge in mouse models of allergic asthma using hyperpolarized 3He MRI.
Article in NMR in biomedicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Lung function measurements in preclinical research: What has been done and where is it headed?Frontiers in physiology · 2023Review
- Preclinical Four-Dimensional Functional Lung Imaging and Quantification of Regional Airflow: A New Standard in Lung Function Evaluation in Murine Models.American journal of respiratory cell and molecular biology · 2022Article
- Drug class effects on respiratory mechanics in animal models: access and applications.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Respiratory mechanics evaluation of mice submitted to intravenous methacholine: Bolus vs. continuous infusion.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- A portable ventilator with integrated physiologic monitoring for hyperpolarizedJournal of magnetic resonance (San Diego, Calif. : 1997) · 2018Article
- Magnetic resonance imaging with hyperpolarized agents: methods and applications.Physics in medicine and biology · 2017Review
- Vagal innervation is required for pulmonary function phenotype inAmerican journal of physiology. Lung cellular and molecular physiology · 2017Article
- Investigating lung responses with functional hyperpolarized xenon-129 MRI in an ex vivo rat model of asthma.Magnetic resonance in medicine · 2016Article
- Hyperpolarized and inert gas MRI: the future.Molecular imaging and biology · 2015Review
- Effects of corticosteroid treatment on airway inflammation, mechanics, and hyperpolarized ³He magnetic resonance imaging in an allergic mouse model.Journal of applied physiology (Bethesda, Md. : 1985) · 2012Article
- A Constant-Volume Ventilator and Gas Recapture System for Hyperpolarized Gas MRI of Mouse and Rat Lungs.Concepts in magnetic resonance. Part B, Magnetic resonance engineering · 2011Article
- Gradient-induced longitudinal relaxation of hyperpolarized noble gases in the fringe fields of superconducting magnets used for magnetic resonance.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2011Article
- Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine.Magnetic resonance in medicine · 2010Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Hyperpolarized (HP) (3)He magnetic resonance imaging has been recently used to produce high-resolution images of pulmonary ventilation after methacholine (MCh) challenge in mouse models of allergic inflammation. This capability presents an opportunity to gain new insights about these models and to more sensitively evaluate new drug treatments in the pre-clinical setting. In the current study, we present our initial experience using two-dimensional (2D), time-resolved (3)He MRI of MCh challenge-induced airways hyperreactivity (AHR) to compare ovalbumin-sensitized and challenged (N = 8) mice to controls (N = 8). Imaging demonstrated that ovalbumin-sensitized and challenged animals exhibited many large ventilation defects even prior to MCh challenge (four out of eight) compared to no defects in the control animals. Additionally, the ovalbumin-sensitized and challenged animals experienced a greater number of ventilation defects (4.5 +/- 0.4) following MCh infusion than did controls (3.3 +/- 0.6). However, due to variability in MCh delivery that was specific to the small animal MRI environment, the difference in mean defect number was not statistically significant. These findings are reviewed in detail and a comprehensive solution to the variability problem is presented that has greatly enhanced the magnitude and reproducibility of the MCh response. This has permitted us to develop a new imaging protocol consisting of a baseline 3D image, a time-resolved 2D series during MCh challenge, and a post-MCh 3D image that reveals persistent ventilation defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.