ReviewNature biotechnology2009
Protein promiscuity and its implications for biotechnology.
Review in Nature biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 197 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
197 citing papers in PubMed.
- Enabling molecular signaling with temperature and ionic-strength independence or programmable dependence.Nature communications · 2026Article
- Dual and opposing roles of the EXD2 exonuclease in the resolution of RNA-DNA hybrids.Nucleic acids research · 2026Article
- Promiscuity-Guided Enzyme Evolution via Substrate Multiplexed Screening.Angewandte Chemie (International ed. in English) · 2026Review
- Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl-tRNA Synthetase Boosts Genetic Code Expansion in E. coli.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Experimental evolution to thermal stress indicates climate resilience in a cosmopolitan arthropod.eLife · 2026Article
- A tri-modal contrastive learning framework for protein representation learning.Cell reports methods · 2026Article
- Genome-wide analysis of flax bZIP genes using a T2T genome reveals abiotic stress roles.iScience · 2026Article
- Multimerization interactions between protein-inspired single-chain random heteropolymers.PloS one · 2026Article
- Antibody Polyreactivity: A Challenger of Immune Paradigms.Immunology · 2025Review
- Identification of PP2C gene family and its role in stress and adversity based on T2T flax (Linum usitatissimum L.) genome.BMC plant biology · 2025Article
- Enzyme promiscuity in the field of synthetic biology applied to white biotechnology: Opportunities and weaknesses.Biodesign research · 2025Review
- DeepMolecules: a web server for predicting enzyme and transporter-small molecule interactions.Nucleic acids research · 2025Article
- Population Thinking and the Uniqueness of Biological Entities.Acta biotheoretica · 2025Article
- Identification and Characterization of WOX Gene Family in Flax (International journal of molecular sciences · 2025Article
- Evolutionary Dynamics of RuBisCO: Emergence of the Small Subunit and its Impact Through Time.Molecular biology and evolution · 2025Article
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- Emergence of power law distributions in protein-protein interaction networks through study bias.eLife · 2024Article
- Predicting therapeutic and side effects from drug binding affinities to human proteome structures.iScience · 2024Article
- Testing the predictive power of reverse screening to infer drug targets, with the help of machine learning.Communications chemistry · 2024Article
137 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular recognition between proteins and their interacting partners underlies the biochemistry of living organisms. Specificity in this recognition is thought to be essential, whereas promiscuity is often associated with unwanted side effects, poor catalytic properties and errors in biological function. Recent experimental evidence suggests that promiscuity, not only in interactions but also in the actual function of proteins, is not as rare as was previously thought. This has implications not only for our fundamental understanding of molecular recognition and how protein function has evolved over time but also in the realm of biotechnology. Understanding protein promiscuity is becoming increasingly important not only to optimize protein engineering applications in areas as diverse as synthetic biology and metagenomics but also to lower attrition rates in drug discovery programs, identify drug interaction surfaces less susceptible to escape mutations and potentiate the power of polypharmacology.
Indexed as
Identifiers
19204698What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.