ArticleThe Journal of cell biology2009
Kinetochore-generated pushing forces separate centrosomes during bipolar spindle assembly.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
85 citing papers in PubMed, 133 citations in OpenAlex.
- FOXM1 Signaling Network Transcriptionally Upregulates Expression of Proteins Involved in Mitotic Progression to Induce High Proliferation and Chromosomal Instability in Androgen Receptor-Low Triple-Negative Breast Cancer.International journal of molecular sciences · 2026Article
- Pre-rRNAs control mitosis by maintaining chromosomal segregation through protecting SMC2 from AURKA-mediated phosphorylation.Cell death & disease · 2025Article
- Kinesin Spindle Protein (KIF11) in Mitosis and Cancer.International journal of molecular sciences · 2025Review
- The LINC complex ensures accurate centrosome positioning during prophase.Life science alliance · 2024Article
- Nucleolin is required for multiple centrosome-associated functions in early vertebrate mitosis.Chromosoma · 2023Article
- Mechanisms underlying spindle assembly and robustness.Nature reviews. Molecular cell biology · 2023Review
- A fine balance among key biophysical factors is required for recovery of bipolar mitotic spindle from monopolar and multipolar abnormalities.Molecular biology of the cell · 2023Article
- Emerging roles of centrosome cohesion.Open biology · 2022Review
- Polar Chromosomes-Challenges of a Risky Path.Cells · 2022Review
- WDR62 localizes katanin at spindle poles to ensure synchronous chromosome segregation.The Journal of cell biology · 2021Article
- Stable kinetochore-microtubule attachments restrict MTOC position and spindle elongation in oocytes.EMBO reports · 2021Article
- Tell the Difference Between Mitosis and Meiosis: Interplay Between Chromosomes, Cytoskeleton, and Cell Cycle Regulation.Frontiers in cell and developmental biology · 2021Review
- Centrosome-nuclear axis repositioning drives the assembly of a bipolar spindle scaffold to ensure mitotic fidelity.Molecular biology of the cell · 2020Article
- Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes.Nature communications · 2020Article
- Centrosomal and Non-centrosomal Functions Emerged through Eliminating Centrosomes.Cell structure and function · 2020Review
- Review
- Nek2-mediated GAS2L1 phosphorylation and centrosome-linker disassembly induce centrosome disjunction.The Journal of cell biology · 2020Article
- Ensemble-Level Organization of Human Kinetochores and Evidence for Distinct Tension and Attachment Sensors.Cell reports · 2020Article
- NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells.The EMBO journal · 2020Article
- Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes.Cell division · 2020Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In animal somatic cells, bipolar spindle formation requires separation of the centrosome-based spindle poles. Centrosome separation relies on multiple pathways, including cortical forces and antiparallel microtubule (MT) sliding, which are two activities controlled by the protein kinase aurora A. We previously found that depletion of the human kinetochore protein Mcm21R(CENP-O) results in monopolar spindles, raising the question as to whether kinetochores contribute to centrosome separation. In this study, we demonstrate that kinetochores promote centrosome separation after nuclear envelope breakdown by exerting a pushing force on the kinetochore fibers (k-fibers), which are bundles of MTs that connect kinetochores to centrosomes. This force is based on poleward MT flux, which incorporates new tubulin subunits at the plus ends of k-fibers and requires stable k-fibers to drive centrosomes apart. This kinetochore-dependent force becomes essential for centrosome separation if aurora A is inhibited. We conclude that two mechanisms control centrosome separation during prometaphase: an aurora A-dependent pathway and a kinetochore-dependent pathway that relies on k-fiber-generated pushing forces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.