ArticleMolecular and cellular biology2009
Sub1 functions in osmoregulation and in transcription by both RNA polymerases II and III.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 41 citations in OpenAlex.
- Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA.Biochemistry · 2023Article
- The Role ofCells · 2022Article
- The Catalytic Subunit ofInternational journal of molecular sciences · 2022Article
- Article
- Glycolytic flux inThe Biochemical journal · 2019Article
- Sub1 and RNAPII, until termination does them part.Transcription · 2018Review
- MPK1/SLT2 Links Multiple Stress Responses with Gene Expression in Budding Yeast by Phosphorylating Tyr1 of the RNAP II CTD.Molecular cell · 2017Article
- Sub1/PC4, a multifaceted factor: from transcription to genome stability.Current genetics · 2017Review
- Sub1 contacts the RNA polymerase II stalk to modulate mRNA synthesis.Nucleic acids research · 2017Article
- Proteomic Analysis of the Mediator Complex Interactome in Saccharomyces cerevisiae.Scientific reports · 2017Article
- Barcode Sequencing Screen Identifies SUB1 as a Regulator of Yeast Pheromone Inducible Genes.G3 (Bethesda, Md.) · 2016Article
- Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA.Chemical communications (Cambridge, England) · 2015Article
- Substitution of tryptophan 89 with tyrosine switches the DNA binding mode of PC4.Scientific reports · 2015Article
- SUB1 Plays a Negative Role during Starvation Induced Sporulation Program in Saccharomyces cerevisiae.PloS one · 2015Article
- Sub1 and Maf1, two effectors of RNA polymerase III, are involved in the yeast quiescence cycle.PloS one · 2014Article
- Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription.Transcription · 2014Review
- RNA polymerase III-specific general transcription factor IIIC contains a heterodimer resembling TFIIF Rap30/Rap74.Nucleic acids research · 2013Article
- From structure to systems: high-resolution, quantitative genetic analysis of RNA polymerase II.Cell · 2013Article
- Sub1 associates with Spt5 and influences RNA polymerase II transcription elongation rate.Molecular biology of the cell · 2012Article
- Novel interactions at the essential N-terminus of poly(A) polymerase that could regulate poly(A) addition in Saccharomyces cerevisiae.FEBS letters · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sub1 is implicated in transcriptional activation, elongation, and mRNA 3'-end formation in budding yeast. To gain more insight into its function, we performed a synthetic genetic array screen with SUB1 that uncovered genetic interactions with genes involved in the high-osmolarity glycerol (HOG) osmoresponse pathway. We find that Sub1 and the HOG pathway are redundant for survival in moderate osmolarity. Chromatin immunoprecipitation analysis shows that Sub1 is recruited to osmoresponse gene promoters during osmotic shock and is required for full recruitment of TBP, TFIIB, and RNA polymerase II (RNAP II) at a subset of these genes. Furthermore, we detect Sub1 at the promoter of every constitutively transcribed RNAP II and, unexpectedly, at every RNAP III gene tested, but not at the RNAP I-transcribed ribosomal DNA promoter. Significantly, deletion of SUB1 reduced levels of promoter-associated RNAP II or III at these genes, but not TBP levels. Together these data suggest that, in addition to a general role in polymerase recruitment at constitutive RNAP II and RNAP III genes, during osmotic shock, Sub1 facilitates osmoresponse gene transcription by enhancing preinitiation complex formation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.