ArticleBiochemistry2009
Kinetic mechanism for single-stranded DNA binding and translocation by Saccharomyces cerevisiae Isw2.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Chromatin remodeling factors Isw2 and Ino80 regulate checkpoint activity and chromatin structure in S phase.Genetics · 2015Article
- Kinetic mechanism of DNA translocation by the RSC molecular motor.Archives of biochemistry and biophysics · 2013Article
- RecQ helicase translocates along single-stranded DNA with a moderate processivity and tight mechanochemical coupling.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Translocation of E. coli RecQ helicase on single-stranded DNA.Biochemistry · 2012Article
- Swi2/Snf2 remodelers: hybrid views on hybrid molecular machines.Current opinion in structural biology · 2012Review
- Fluorescence methods to study DNA translocation and unwinding kinetics by nucleic acid motors.Methods in molecular biology (Clifton, N.J.) · 2012Article
- The macroscopic rate of nucleic acid translocation by hepatitis C virus helicase NS3h is dependent on both sugar and base moieties.Journal of molecular biology · 2010Article
- Processive translocation mechanism of the human Bloom's syndrome helicase along single-stranded DNA.Nucleic acids research · 2010Article
- Streamlined determination of processive run length and mechanochemical coupling of nucleic acid motor activities.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The chromatin remodeling complex Isw2 from Saccharomyces cerevisiae (yIsw2) mobilizes nucleosomes through an ATP-dependent reaction that is coupled to the translocation of the helicase domain of the enzyme along intranucleosomal DNA. In this study, we demonstrate that yIsw2 is capable of translocating along single-stranded DNA in a reaction that is coupled to ATP hydrolysis. We propose that single-stranded DNA translocation by yIsw2 occurs through a series of repeating uniform steps with an overall macroscopic processivity (P) of 0.90 +/- 0.02, corresponding to an average translocation distance of 20 +/- 2 nucleotides before dissociation. This processivity corresponds well to the processivity of nucleosome sliding by yIsw2, thus arguing that single-stranded DNA translocation or tracking may be fundamental to the double-stranded DNA translocation required for effective nucleosome mobilization. Furthermore, we find evidence that a slow initiation process, following DNA binding, may be required to make yIsw2 competent for DNA translocation. We also provide evidence that this slow initiation process may correspond to the second step of a two-step DNA binding mechanism by yIsw2 and a quantitative description of the kinetics of this DNA binding mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.