ArticleApplied and environmental microbiology2009
Reciprocal expression of integration host factor and HU in the developmental cycle and infectivity of Legionella pneumophila.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Transcription factors DksA and PsrA are synergistic contributors toMicrobiology (Reading, England) · 2025Article
- New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila.Molecular & cellular proteomics : MCP · 2022Article
- Development of a Fluorescent Tool for StudyingMicroorganisms · 2021Article
- Differential Bacterial Predation by Free-Living Amoebae May Result in Blooms ofMicroorganisms · 2021Article
- The Temporal Expression of Global Regulator Protein CsrA Is Dually Regulated by ClpP During the Biphasic Life Cycle ofFrontiers in microbiology · 2019Article
- Defining the Functionally Important Domain and Amino Acid Residues in Mycobacterium tuberculosis Integration Host Factor for Genome Stability, DNA Binding, and Integrative Recombination.Journal of bacteriology · 2017Article
- Legionella pneumophila OxyR Is a Redundant Transcriptional Regulator That Contributes to Expression Control of the Two-Component CpxRA System.Journal of bacteriology · 2017Article
- Identification of vacuoles containing extraintestinal differentiated forms of Legionella pneumophila in colonized Caenorhabditis elegans soil nematodes.MicrobiologyOpen · 2015Article
- Regulation of bacterial virulence by Csr (Rsm) systems.Microbiology and molecular biology reviews : MMBR · 2015Review
- A regulatory feedback loop between RpoS and SpoT supports the survival of Legionella pneumophila in water.Applied and environmental microbiology · 2015Article
- Two Fis regulators directly repress the expression of numerous effector-encoding genes in Legionella pneumophila.Journal of bacteriology · 2014Article
- The many forms of a pleomorphic bacterial pathogen-the developmental network of Legionella pneumophila.Frontiers in microbiology · 2014Review
- Disulfide bond oxidoreductase DsbA2 of Legionella pneumophila exhibits protein disulfide isomerase activity.Journal of bacteriology · 2013Article
- The nucleoid-associated protein HUβ affects global gene expression in Porphyromonas gingivalis.Microbiology (Reading, England) · 2013Article
- Structure of RdxA--an oxygen-insensitive nitroreductase essential for metronidazole activation in Helicobacter pylori.The FEBS journal · 2012Article
- EbfC (YbaB) is a new type of bacterial nucleoid-associated protein and a global regulator of gene expression in the Lyme disease spirochete.Journal of bacteriology · 2012Article
- Aberrant community architecture and attenuated persistence of uropathogenic Escherichia coli in the absence of individual IHF subunits.PloS one · 2012Article
- DsbA2 (27 kDa Com1-like protein) of Legionella pneumophila catalyses extracytoplasmic disulphide-bond formation in proteins including the Dot/Icm type IV secretion system.Molecular microbiology · 2011Article
- Legionella pneumophila LbtU acts as a novel, TonB-independent receptor for the legiobactin siderophore.Journal of bacteriology · 2011Article
- The Legionella pneumophila Chaperonin - An Unusual Multifunctional Protein in Unusual Locations.Frontiers in microbiology · 2011Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Legionella pneumophila is an intracellular parasite of protozoa that differentiates late in infection into metabolically dormant cysts that are highly infectious. Regulation of this process is poorly understood. Here we report that the small DNA binding regulatory proteins integration host factor (IHF) and HU are reciprocally expressed over the developmental cycle, with HU expressed during exponential phase and IHF expressed postexponentially. To assess the role of these regulatory proteins in development, chromosomal deletions were constructed. Single (ihfA or ihfB) and double deletion (Deltaihf) IHF mutants failed to grow in Acanthamoeba castellanii unless complemented in trans when expressed temporally from the ihfA promoter but not under P(tac) (isopropyl-beta-d-thiogalactopyranoside). In contrast, IHF mutants were infectious for HeLa cells, though electron microscopic examination revealed defects in late-stage cyst morphogenesis (thickened cell wall, intracytoplasmic membranes, and inclusions of poly-beta-hydroxybutyrate), and were depressed for the developmental marker MagA. Green fluorescent protein promoter fusion assays indicated that IHF and the stationary-phase sigma factor RpoS were required for full postexponential expression of magA. Finally, defects in cyst morphogenesis noted for Deltaihf mutants in HeLa cells correlated with a loss of both detergent resistance and hyperinfectivity compared with results for wild-type cysts. These studies establish IHF and HU as markers of developmental stages and show that IHF function is required for both differentiation and full virulence of L. pneumophila in natural amoebic hosts.
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