ArticleApplied and environmental microbiology2009
High-throughput bioluminescence-based mutant screening strategy for identification of bacterial virulence genes.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
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- Transposon mutagenesis and identification of mutated genes in growth-delayed Edwardsiella ictaluri.BMC microbiology · 2019Article
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- Identification of Salmonella enterica serovar Kentucky genes involved in attachment to chicken skin.BMC microbiology · 2016Article
- Intramacrophage Infection Reinforces the Virulence of Edwardsiella tarda.Journal of bacteriology · 2016Article
- The contribution of the glycine cleavage system to the pathogenesis of Francisella tularensis.Microbes and infection · 2014Article
- Tricarboxylic acid cycle and one-carbon metabolism pathways are important in Edwardsiella ictaluri virulence.PloS one · 2013Article
- Use of fluorescent immuno-chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples.Journal of visualized experiments : JoVE · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
A high-throughput bioluminescence screening procedure for identification of virulence genes in bacteria was developed and applied to the fish pathogen Edwardsiella ictaluri. A random transposon mutant library expressing bioluminescence was constructed and robotically arrayed on 384-well plates. Mutants were cultivated and mixed with catfish serum and neutrophils in 96-well plates, and bioluminescence was used to detect mutants that are more susceptible to killing by these host factors. The virulence and vaccine efficacy of selected mutants were determined in channel catfish. Transposon insertion sites in 13 mutants attenuated in the natural host were mapped to the E. ictaluri genome. Ten unique genes were mutated, including genes encoding a negative regulator of sigmaE activity, a glycine cleavage system protein, tricarboxylic acid cycle enzymes, an O polysaccharide biosynthesis enzyme, proteins encoded on the native plasmid pEI1, and a fimbrial chaperon protein. Three of these mutants were found to have potential as live attenuated vaccines. This study demonstrates a novel application of bioluminescence to identify bacterial genes required for host resistance; as a result, efficacious and genetically defined live attenuated vaccine candidates were developed.
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Registered trials
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