ArticleAmerican journal of physiology. Lung cellular and molecular physiology2009
Ozone modulates IL-6 secretion in human airway epithelial and smooth muscle cells.
Article in American journal of physiology. Lung cellular and molecular physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Inflammatory and repair pathways induced in human bronchoalveolar lavage cells with ozone inhalation.PloS one · 2015Trial
- Single-Cell RNA Sequencing Identifies a Unique Macrophage Population in a Mouse Model of Ozone-induced Asthma Exacerbation.American journal of respiratory cell and molecular biology · 2025Article
- Modulation of airway hyperresponsiveness by rhinovirus exposure.Respiratory research · 2018Review
- Roles of airway smooth muscle dysfunction in chronic obstructive pulmonary disease.Journal of translational medicine · 2018Review
- Augmented Pla2g4c/Ptgs2/Hpgds axis in bronchial smooth muscle tissues of experimental asthma.PloS one · 2018Article
- Formaldehyde Induces Rho-Associated Kinase Activity to Evoke Airway Hyperresponsiveness.American journal of respiratory cell and molecular biology · 2016Article
- Assessment of biological responses of EpiAirway 3-D cell constructs versus A549 cells for determining toxicity of ambient air pollution.Inhalation toxicology · 2016Article
- Unjamming and cell shape in the asthmatic airway epithelium.Nature materials · 2015Article
- Pivotal role of IL-6 in the hyperinflammatory responses to subacute ozone in adiponectin-deficient mice.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Trichostatin A abrogates airway constriction, but not inflammation, in murine and human asthma models.American journal of respiratory cell and molecular biology · 2012Article
- Targeting airway smooth muscle in airways diseases: an old concept with new twists.Expert review of respiratory medicine · 2011Review
- Does airway smooth muscle express an inflammatory phenotype in asthma?British journal of pharmacology · 2011Review
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- Inhibition of myristoylated alanine-rich C kinase substrate (MARCKS) protein inhibits ozone-induced airway neutrophilia and inflammation.Experimental lung research · 2010Article
- Lasers, stem cells, and COPD.Journal of translational medicine · 2010Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Although ozone enhances leukocyte function and recruitment in airways, the direct effect of ozone in modulating structural cell-derived inflammatory mediators remains unknown. Using a coculture model comprised of differentiated human airway epithelial cells (NHBE) and smooth muscle cells (ASM), we postulate that ozone regulates IL-6 secretion in basal and cytokine-primed structural cells. Air-liquid interface (ALI) cultures of NHBE cells underwent differentiation as determined by mucin secretion, transepithelial electrical resistance (TEER), and ultrastructure parameters. Whereas TNF enhanced basal secretion of IL-6 (57 +/- 3%), ozone exposure at 0.6 ppm for 6 h augmented IL-6 levels in basal (41 +/- 3%) and TNF- (50 +/- 5%) primed cocultures compared with that derived from NHBE or ASM monolayers alone. Levels of PGE(2), 6-keto-PGF(1alpha), PGF(2alpha), and thromboxane B(2) (TxB(2)) levels in basal and TNF-primed cocultures revealed that ozone selectively enhanced PGE(2) production in TNF- (6 +/- 3-fold) primed cocultures, with little effect (P > 0.05) on diluent-treated cultures. In accordance with ozone-induced increases in PGE(2) levels, cyclooxygenase inhibition with indomethacin partially abolished IL-6 secretion. Surprisingly, indomethacin had little effect on constitutive secretion of IL-6 in cocultures, whereas indomethacin completely restored ozone-mediated TEER reduction in TNF-primed cocultures. Collectively, our data for the first time suggest a dual role of ozone in modulating IL-6 secretion and TEER outcomes in a PGE(2)-dependent (in presence of TNF stimulus) and -independent manner (in absence of cytokine stimulus).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.