ArticleJournal of bacteriology2009
Sporulation and enterotoxin (CPE) synthesis are controlled by the sporulation-specific sigma factors SigE and SigK in Clostridium perfringens.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 112 citations in OpenAlex.
- Milestones in Clostridium perfringens research since 1995.Anaerobe · 2025Review
- The biology and pathogenicity ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- Spores of Clostridioides difficile are toxin delivery vehicles.Communications biology · 2024Article
- The Effect of Caco-2 Cells on Sporulation and Enterotoxin Expression by FoodbornePathogens (Basel, Switzerland) · 2024Article
- Overexpressing theToxins · 2024Article
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- Do Bacteria Provide an Alternative to Cancer Treatment and What Role Does Lactic Acid Bacteria Play?Microorganisms · 2022Review
- Characterization of Putative Sporulation and Germination Genes inMicroorganisms · 2022Article
- Article
- Review
- Pathogenicity and virulence ofVirulence · 2021Article
- Article
- Characteristics of the sigK Deletion Mutant from Bacillus thuringiensis var. israelensis Strain Bt-59.Current microbiology · 2020Article
- Changes in the expression of genes encoding type IV pili-associated proteins are seen when Clostridium perfringens is grown in liquid or on surfaces.BMC genomics · 2020Article
- Sporulation and Germination in Clostridial Pathogens.Microbiology spectrum · 2019Review
- Article
- NanR Regulates Sporulation and Enterotoxin Production by Clostridium perfringens Type F Strain F4969.Infection and immunity · 2018Article
- Enterotoxic Clostridia:Microbiology spectrum · 2018Article
- An update on the human and animal enteric pathogen Clostridium perfringens.Emerging microbes & infections · 2018Review
- Effects of Bile Acids and Nisin on the Production of Enterotoxin byInternational journal of microbiology · 2018Article
4 more citing papers are in PubMed but not listed here.
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4 authors at 2 institutions in 1 country.
Funding
Abstract
Clostridium perfringens is the third most frequent cause of bacterial food poisoning annually in the United States. Ingested C. perfringens vegetative cells sporulate in the intestinal tract and produce an enterotoxin (CPE) that is responsible for the symptoms of acute food poisoning. Studies of Bacillus subtilis have shown that gene expression during sporulation is compartmentalized, with different genes expressed in the mother cell and the forespore. The cell-specific RNA polymerase sigma factors sigma(F), sigma(E), sigma(G), and sigma(K) coordinate much of the developmental process. The C. perfringens cpe gene, encoding CPE, is transcribed from three promoters, where P1 was proposed to be sigma(K) dependent, while P2 and P3 were proposed to be sigma(E) dependent based on consensus promoter recognition sequences. In this study, mutations were introduced into the sigE and sigK genes of C. perfringens. With the sigE and sigK mutants, gusA fusion assays indicated that there was no expression of cpe in either mutant. Results from gusA fusion assays and immunoblotting experiments indicate that sigma(E)-associated RNA polymerase and sigma(K)-associated RNA polymerase coregulate each other's expression. Transcription and translation of the spoIIID gene in C. perfringens were not affected by mutations in sigE and sigK, which differs from B. subtilis, in which spoIIID transcription requires sigma(E)-associated RNA polymerase. The results presented here show that the regulation of developmental events in the mother cell compartment of C. perfringens is not the same as that in B. subtilis and Clostridium acetobutylicum.
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