ArticleThe Journal of biological chemistry2009
Role of focal adhesion kinase Ser-732 phosphorylation in centrosome function during mitosis.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- A comparative analysis of paxillin and Hic-5 proximity interactomes.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Bystanders or active players: the role of extra centrosomes as signaling hubs.Cancer metastasis reviews · 2024Review
- The Endothelial Centrosome: Specific Features and Functional Significance for Endothelial Cell Activity and Barrier Maintenance.International journal of molecular sciences · 2023Review
- Article
- Contribution of integrin adhesion to cytokinetic abscission and genomic integrity.Frontiers in cell and developmental biology · 2022Review
- Inositol hexakisphosphate kinase 3 promotes focal adhesion turnover via interactions with dynein intermediate chain 2.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Review
- Paxillin regulates cell polarization and anterograde vesicle trafficking during cell migration.Molecular biology of the cell · 2017Article
- Antitumor Effect of KX-01 through Inhibiting Src Family Kinases and Mitosis.Cancer research and treatment · 2017Article
- Insights into the clinical value of cyclin-dependent kinase 5 in glioma: a retrospective study.World journal of surgical oncology · 2015Article
- Article
- Centriole biogenesis and function in multiciliated cells.Methods in cell biology · 2015Article
- CDK5 is a major regulator of the tumor suppressor DLC1.The Journal of cell biology · 2014Article
- Endothelial deletion of Sag/Rbx2/Roc2 E3 ubiquitin ligase causes embryonic lethality and blocks tumor angiogenesis.Oncogene · 2014Article
- CDK5 is essential for TGF-β1-induced epithelial-mesenchymal transition and breast cancer progression.Scientific reports · 2013Article
- EGFR/MEK/ERK/CDK5-dependent integrin-independent FAK phosphorylated on serine 732 contributes to microtubule depolymerization and mitosis in tumor cells.Cell death & disease · 2013Article
- The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.Cell cycle (Georgetown, Tex.) · 2013Article
- Class 3 semaphorin mediates dendrite growth in adult newborn neurons through Cdk5/FAK pathway.PloS one · 2013Article
- Increased asymmetric and multi-daughter cell division in mechanically confined microenvironments.PloS one · 2012Article
- How focal adhesion kinase achieves regulation by linking ligand binding, localization and action.Current opinion in structural biology · 2011Review
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Authors and funding
4 authors.
Funding
Abstract
Focal adhesion kinase (FAK) is the major cytoplasmic tyrosine kinase in focal adhesions and a critical mediator of integrin signaling in a variety of cells, including endothelial cells (ECs). Here we describe a new function for FAK in the regulation of centrosome functions in a Ser-732 phosphorylation-dependent manner during mitosis. Deletion of FAK in primary ECs causes increases in centrosome numbers, multipolar and disorganized spindles, and unaligned chromosomes during mitosis. Re-expression of wild-type FAK, but not S732A mutant, rescued these mitotic defects, suggesting a role for Ser-732 phosphorylation in the regulation of centrosomal functions. Consistent with this possibility, Ser-732-phosphorylated FAK was found to co-localize in centrosomes in mitotic cells. FAK also associated with cytoplasmic dynein in a Ser-732 phosphorylation-dependent manner. Further analysis in FAK-null primary ECs showed that S732A mutant could rescue EC migration but not proliferation or tubulogenesis in vitro. Last, we showed that deletion of FAK in ECs reduced tumor angiogenesis in vivo, which could be restored by re-expression of wild-type FAK but not S732A mutant. Together, these studies demonstrated a novel role for Ser-732 phosphorylation of FAK in the regulation of centrosome function during mitosis, which may contribute to EC proliferation and angiogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.