ArticleBiomaterials2009
Sustained transgene expression via citric acid-based polyester elastomers.
Article in Biomaterials, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 62 citations in OpenAlex.
- Trivalent ions kinetic-gating for producing high-concentration and shelf-stable plasmid DNA/PEI particles.Nature communications · 2026Article
- Biomimetic fabricated tubular graftBioactive materials · 2025Article
- Citric Acid: A Nexus Between Cellular Mechanisms and Biomaterial Innovations.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Engineering multifunctional bioactive citrate-based biomaterials for tissue engineering.Bioactive materials · 2023Review
- Enhanced wound healing via collagen-turnover-driven transfer of PDGF-BB gene in a murine wound model.ACS applied bio materials · 2020Article
- Biomaterial-based delivery systems of nucleic acid for regenerative research and regenerative therapy.Regenerative therapy · 2019Review
- ECM turnover-stimulated gene delivery through collagen-mimetic peptide-plasmid integration in collagen.Acta biomaterialia · 2017Article
- Citrate-Based Biomaterials and Their Applications in Regenerative Engineering.Annual review of materials research · 2015Article
- A biodegradable thermoset polymer made by esterification of citric acid and glycerol.Journal of biomedical materials research. Part A · 2014Article
- Extracellular matrix production in vitro in cartilage tissue engineering.Journal of translational medicine · 2014Review
- Sustained, localized transgene expression mediated from lentivirus-loaded biodegradable polyester elastomers.Journal of biomedical materials research. Part A · 2013Article
- Engineering therapies in the CNS: what works and what can be translated.Neuroscience letters · 2012Review
- Hydrogels to modulate lentivirus delivery in vivo from microporous tissue engineering scaffolds.Drug delivery and translational research · 2011Article
- The contribution of plasmid design and release to in vivo gene expression following delivery from cationic polymer modified scaffolds.Biomaterials · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Polymeric scaffolds are an important tool in tissue engineering and gene delivery using porous scaffolds can be a viable approach to control tissue response. Herein we describe the use of a biodegradable polyester elastomer, poly(1,8-octanediol-co-citrate) (POC), as a substrate for plasmid immobilization and cellular transfection of colonizing cells. Plasmid (pDNA), either complexed with poly(ethyleneimine) (PEI) forming polyplexes or in its native state, was surface-immobilized onto POC scaffolds via adsorption. Polyplex-containing scaffolds showed higher loading and slower initial rates of release than naked pDNA-containing scaffolds. Seeding of HEK293 cells and porcine aortic smooth muscle cells (PASMC) onto polyplex loaded-scaffolds demonstrated cell proliferation and transfection in vitro up to 12 days, significantly longer relative to bolus transfection. In vivo, transfection was evaluated using the mouse intraperitoneal (IP) fat model. In contrast to the in vitro study, successful long-term transgene delivery was only achieved with the naked pDNA-containing scaffolds. In particular, naked pDNA-containing scaffolds promoted high levels of both luciferase and green fluorescent protein (GFP) expression in vivo for 2 weeks. The results demonstrate that POC scaffolds are a suitable material for substrate-mediated gene delivery. POC scaffolds can potentially support long-term biological cues to mediate tissue formation through non-viral gene delivery.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.