ArticleBMC microbiology2009
Secretion of flagellin by the LEE-encoded type III secretion system of enteropathogenic Escherichia coli.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- First report on the physicochemical and proteomic characterization ofFrontiers in microbiology · 2024Article
- Evidence of Naturalized Stress-Tolerant Strains of Escherichia coli in Municipal Wastewater Treatment Plants.Applied and environmental microbiology · 2016Article
- Use of a Novel Report Protein to Study the Secretion Signal of Flagellin in Bacillus subtilis.Current microbiology · 2016Article
- The Type VI Secretion System Modulates Flagellar Gene Expression and Secretion in Citrobacter freundii and Contributes to Adhesion and Cytotoxicity to Host Cells.Infection and immunity · 2015Article
- Secretion of Flagellar Proteins by the Pseudomonas aeruginosa Type III Secretion-Injectisome System.Journal of bacteriology · 2015Article
- Salmonella enterica serovar enteritidis antimicrobial peptide resistance genes aid in defense against chicken innate immunity, fecal shedding, and egg deposition.Infection and immunity · 2014Article
- Assembly of the type II secretion system such as found in Vibrio cholerae depends on the novel Pilotin AspS.PLoS pathogens · 2013Article
- Mutations that impact the enteropathogenic Escherichia coli Cpx envelope stress response attenuate virulence in Galleria mellonella.Infection and immunity · 2012Article
- The Campylobacter jejuni CiaC virulence protein is secreted from the flagellum and delivered to the cytosol of host cells.Frontiers in cellular and infection microbiology · 2012Article
- Article
- A type III effector protease NleC from enteropathogenic Escherichia coli targets NF-κB for degradation.Molecular microbiology · 2011Article
- Amino-terminal residues dictate the export efficiency of the Campylobacter jejuni filament proteins via the flagellum.Molecular microbiology · 2010Article
- Identification of a Campylobacter jejuni-secreted protein required for maximal invasion of host cells.Molecular microbiology · 2009Article
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Authors and funding
5 authors.
Funding
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Abstract
backgroundEnteropathogenic Escherichia coli (EPEC) is an attaching and effacing (A/E) pathogen that possesses a type III secretion system (T3SS) encoded within the locus of enterocyte effacement (LEE). The LEE is essential for A/E lesion formation and directs the secretion and translocation of multiple LEE-encoded and non-LEE encoded effector proteins into the cytosol of infected cells. In this study we used proteomics to compare proteins exported to the culture supernatant by wild type EPEC E2348/69, a DeltaespADB mutant and a DeltaescF T3SS mutant.
resultsWe observed that flagellin was consistently and strongly present in the secretome of wild type EPEC and the DeltaespADB mutant but present only weakly in the secretome of the DeltaescF mutant. Given the ancestral relationship between the flagella export apparatus and virulence associated T3SSs, we investigated whether FliC could utilise the LEE-encoded T3SS for export. In the absence of a functional flagella export apparatus, we showed that FliC could be secreted by the LEE-encoded T3SS and stimulate (Toll-like receptor 5) TLR5 signalling but could not confer motility.
conclusionSince the secretion of FliC during A/E lesion formation would presumably be disadvantageous for the pathogen, we propose that virulence associated T3SSs and flagella T3SSs have evolved through a system of chaperones and complex regulatory pathways to be functional at different times to ensure that FliC secretion does not occur during T3SS effector translocation.
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