ArticleReproductive biology and endocrinology : RB&E2009
Reduced levels of intracellular calcium releasing in spermatozoa from asthenozoospermic patients.
Article in Reproductive biology and endocrinology : RB&E, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 70 citations in OpenAlex.
- A comprehensive review about sperm-oocyte interactions and key activation factors of fertilization in mammals.The Canadian journal of urology · 2026Review
- Impact of Type 1 Diabetes on Testicular Microtubule Dynamics, Sperm Physiology, and Male Reproductive Health in Rat.International journal of molecular sciences · 2025Article
- Voltage-Dependent Anion Channels in Male Reproductive Cells: Players in Healthy Fertility?Biomolecules · 2024Review
- Effects of in vitro repaglinide supplementation on improving sperm motility parameters, viability, and DNA integrity in normozoospermic and asthenozoospermic men.JBRA assisted reproduction · 2023Article
- Mitochondria Quality Control and Male Fertility.Biology · 2023Review
- Testicular aging, male fertility and beyond.Frontiers in endocrinology · 2022Review
- Occurrence of Calcium Oscillations in Human Spermatozoa Is Based on Spatial Signaling Enzymes Distribution.International journal of molecular sciences · 2021Article
- The Modulation of Functional Status of Bovine Spermatozoa by Progesterone.Animals : an open access journal from MDPI · 2021Article
- Role of sperm apoptosis and oxidative stress in male infertility: A narrative review.International journal of reproductive biomedicine · 2021Review
- PGRMC1 Inhibits Progesterone-Evoked Proliferation and CaInternational journal of molecular sciences · 2020Article
- Dissecting the signaling pathways involved in the function of sperm flagellum.Current opinion in cell biology · 2020Review
- Human sperm ion channel (dys)function: implications for fertilization.Human reproduction update · 2019Article
- Apoptosis Is a Demanding Selective Tool During the Development of Fetal Male Germ Cells.Frontiers in cell and developmental biology · 2018Review
- Signaling mechanisms in mammalian sperm motility.Biology of reproduction · 2017Review
- Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility.Scientific reports · 2016Article
- Regulation and roles of Ca2+ stores in human sperm.Reproduction (Cambridge, England) · 2015Review
- Epilepsy but not mobile phone frequency (900 MHz) induces apoptosis and calcium entry in hippocampus of epileptic rat: involvement of TRPV1 channels.The Journal of membrane biology · 2015Article
- Electromagnetic radiation (Wi-Fi) and epilepsy induce calcium entry and apoptosis through activation of TRPV1 channel in hippocampus and dorsal root ganglion of rats.Metabolic brain disease · 2014Article
- Calcium- and integrin-binding protein-1 is down-regulated in the sperm of patients with oligoasthenozoospermia : CIB1 expression in patients with oligoasthenozoospermia.Journal of assisted reproduction and genetics · 2014Article
- Hypericum perforatum modulates apoptosis and calcium mobilization through voltage-gated and TRPM2 calcium channels in neutrophil of patients with Behcet's disease.The Journal of membrane biology · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAsthenozoospermia is one of the most common findings present in infertile males characterized by reduced or absent sperm motility, but its aetiology remains unknown in most cases. In addition, calcium is one of the most important ions regulating sperm motility. In this study we have investigated the progesterone-evoked intracellular calcium signal in ejaculated spermatozoa from men with normospermia or asthenozoospermia.
methodsHuman ejaculates were obtained from healthy volunteers and asthenospermic men by masturbation after 4-5 days of abstinence. For determination of cytosolic free calcium concentration, spermatozoa were loaded with the fluorescent ratiometric calcium indicator Fura-2.
resultsTreatment of spermatozoa from normospermic men with 20 micromolar progesterone plus 1 micromolar thapsigargin in a calcium free medium induced a typical transient increase in cytosolic free calcium concentration due to calcium release from internal stores. Similar results were obtained when spermatozoa were stimulated with progesterone alone. Subsequent addition of calcium to the external medium evoked a sustained elevation in cytosolic free calcium concentration indicative of capacitative calcium entry. However, when progesterone plus thapsigargin were administered to spermatozoa from patients with asthenozoospermia, calcium signal and subsequent calcium entry was much smaller compared to normospermic patients. As expected, pretreatment of normospermic spermatozoa with both the anti-progesterone receptor c262 antibody and with progesterone receptor antagonist RU-38486 decreased the calcium release induced by progesterone. Treatment of spermatozoa with cytochalasin D or jasplakinolide decreased the calcium entry evoked by depletion of internal calcium stores in normospermic patients, whereas these treatments proved to be ineffective at modifying the calcium entry in patients with asthenozoospermia.
conclusionOur results suggest that spermatozoa from asthenozoospermic patients present a reduced responsiveness to progesterone.
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