ArticleJournal of the American Chemical Society2009
Surface-enhanced Raman scattering based ligase detection reaction.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 90 citations in OpenAlex.
- Fluorescence-Based Detection ofMethods and protocols · 2025Article
- Nucleotide Identification in DNA Using Dielectrophoresis Spectroscopy.Micromachines · 2019Article
- Single-Step FRET-Based Detection of Femtomoles DNA.Sensors (Basel, Switzerland) · 2019Article
- DNA Methylation Detection Using Resonance and Nanobowtie-Antenna-Enhanced Raman Spectroscopy.Biophysical journal · 2018Article
- Simultaneous multiple single nucleotide polymorphism detection based on click chemistry combined with DNA-encoded probes.Chemical science · 2018Article
- Single Nucleotide Polymorphism Genotyping in Single-Molecule Electronic Circuits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7.Journal of visualized experiments : JoVE · 2017Article
- Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications.Mutation research. Reviews in mutation research · 2017Review
- Multiparametric Magneto-fluorescent Nanosensors for the Ultrasensitive Detection of Escherichia coli O157:H7.ACS infectious diseases · 2016Article
- Single quantum dot analysis enables multiplexed point mutation detection by gap ligase chain reaction.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
- Immunomagnetic nanoparticle-based assays for detection of biomarkers.International journal of nanomedicine · 2013Review
- Microfluidics in macro-biomolecules analysis: macro inside in a nano world.Analytical and bioanalytical chemistry · 2010Review
- Aptamer based surface enhanced Raman scattering detection of vasopressin using multilayer nanotube arrays.Biosensors & bioelectronics · 2010Article
- A method for nanofluidic device prototyping using elastomeric collapse.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Genomics provides a comprehensive view of the complete genetic makeup of an organism. Individual sequence variations, as manifested by single nucleotide polymorphisms (SNPs), can provide insight into the basis for a large number of phenotypes and diseases including cancer. The ability rapidly screen for SNPs will have a profound impact on a number of applications, most notably personalized medicine. Here we demonstrate a new approach to SNP detection through the application of surface-enhanced Raman scattering (SERS) to the ligase detection reaction (LDR). The reaction uses two LDR primers, one of which contains a Raman enhancer and the other a reporter dye. In LDR, one of the primers is designed to interrogate the SNP. When the SNP being interrogated matches the discriminating primer sequence, the primers are ligated and the enhancer and dye are brought into close proximity enabling the dye's Raman signature to be detected. By detecting the Raman signature of the dye rather than its fluorescence emission, our technique avoids the problem of spectral overlap which limits number of reactions which can be carried out in parallel by existing systems. We demonstrate the LDR-SERS reaction for the detection of point mutations in the human K-ras oncogene. The reaction is implemented in an electrokinetically active microfluidic device that enables physical concentration of the reaction products for enhanced detection sensitivity and quantization. We report a limit of detection of 20 pM of target DNA with the anticipated specificity engendered by the LDR platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.