ArticleAnalytical chemistry2009
Top-down protein identification/characterization of a priori unknown proteins via ion trap collision-induced dissociation and ion/ion reactions in a quadrupole/time-of-flight tandem mass spectrometer.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Gas-Phase Oxidation of Neutral Basic Residues in Polypeptide Cations by Periodate.Journal of the American Society for Mass Spectrometry · 2016Article
- The dehydroalanine effect in the fragmentation of ions derived from polypeptides.Journal of mass spectrometry : JMS · 2016Article
- Oxidation of methionine residues in polypeptide ions via gas-phase ion/ion chemistry.Journal of the American Society for Mass Spectrometry · 2014Article
- Solution versus gas-phase modification of peptide cations with NHS-ester reagents.Journal of the American Society for Mass Spectrometry · 2012Article
- Analysis of intact protein isoforms by mass spectrometry.The Journal of biological chemistry · 2011Review
- Intra- and inter-molecular cross-linking of peptide ions in the gas phase: reagents and conditions.Journal of the American Society for Mass Spectrometry · 2011Article
- Covalent modification of gaseous peptide ions with N-hydroxysuccinimide ester reagent ions.Journal of the American Chemical Society · 2010Article
- Top-down protein characterization facilitated by ion/ion reactions on a quadrupole/time of flight platform.Proteomics · 2010Article
- Reproducible microwave-assisted acid hydrolysis of proteins using a household microwave oven and its combination with LC-ESI MS/MS for mapping protein sequences and modifications.Journal of the American Society for Mass Spectrometry · 2010Article
- Multi-dimensional liquid chromatography in proteomics--a review.Analytica chimica acta · 2010Review
- Gas-phase chemistry of multiply charged bioions in analytical mass spectrometry.Annual review of analytical chemistry (Palo Alto, Calif.) · 2010Review
- Ion/ion reactions: new chemistry for analytical MS.Analytical chemistry · 2009Article
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Authors and funding
3 authors.
Funding
Abstract
The identification and characterization of a priori unknown proteins from an Escherichia coli (E. coli) soluble protein lysate using ion trap collision-induced dissociation of intact protein ions followed by ion/ion reactions in a quadrupole/time-of-flight tandem mass spectrometer is illustrated. The procedure involved the submission of uninterpreted product ion spectra to a peak-picking program and then to ProSightPTM for searching against an E. coli database. Examples are provided for the identification and characterization of both modified and unmodified unknown proteins with masses up to approximately 28 kDa. The availability of protein intact mass along with sequence information makes possible the characterization of proteins with post-translational modifications, such as disulfide linkages, as well as protein isoforms whose sequences are absent from a database, provided that a related form of the gene product is present in the database. This work demonstrates that the quadrupole/time-of-flight platform, in conjunction with ion-ion proton transfer reactions, can be adapted to obtain primary structure information from entire protein ions, rather than simply N- or C-terminal information from low mass-to-charge products, for proteins as large as several tens of kilodaltons.
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