ArticleNaunyn-Schmiedeberg's archives of pharmacology2009
Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors.
Article in Naunyn-Schmiedeberg's archives of pharmacology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
67 citing papers in PubMed, 1 synthesis or guideline pooled it, 153 citations in OpenAlex.
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- Adrenoceptors in the Eye - Physiological and Pathophysiological Relevance.Handbook of experimental pharmacology · 2024Review
- CP-25 inhibits the hyperactivation of rheumatic synoviocytes by suppressing the switch in GCell communication and signaling : CCS · 2023Article
- β-Adrenoreceptors as Therapeutic Targets for Ocular Tumors and Other Eye Diseases-Historical Aspects and Nowadays Understanding.International journal of molecular sciences · 2023Review
- Radioligand Binding to Quantify Adrenergic Receptor Expression in the Heart.Current protocols · 2023Article
- Lifelong changes of neurotransmitter receptor expression and debilitation of hippocampal synaptic plasticity following early postnatal blindness.Scientific reports · 2022Article
- Detection of genome-edited and endogenously expressed G protein-coupled receptors.The FEBS journal · 2021Review
- The Role of Adrenoceptors in the Retina.Cells · 2020Review
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- A novel postsynaptic signal pathway of sympathetic neural regulation of murine colonic motility.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Norepinephrine Has Dual Effects on Human Colonic Contractions Through Distinct Subtypes of Alpha 1 Adrenoceptors.Cellular and molecular gastroenterology and hepatology · 2020Article
- Cardiac βBritish journal of pharmacology · 2019Review
- On the Necessity of Validating Antibodies in the Immunohistochemistry Literature.Frontiers in neuroanatomy · 2019Article
- The Discovery and Development of Liraglutide and Semaglutide.Frontiers in endocrinology · 2019 · on this mapReview
- Nociceptin/Orphanin FQ (N/OFQ) conjugated to ATTO594: a novel fluorescent probe for the N/OFQ (NOP) receptor.British journal of pharmacology · 2018Article
- Glucagon-like peptide-1 receptor expression in the human eye.Diabetes, obesity & metabolism · 2018Article
- Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M(1-4) muscarinic receptor subtypes and alpha(2B)-, beta(2)-, and beta(3)-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.